Yang Yu, Hu Tingting, Zhao Kexin, Wang Yi-Chi, Zhu Yanfang, Wang Shibo, Zhou Zhan, Gu Lin, Tan Chaoliang, Liang Ruizheng
State Key Laboratory of Chemical Resource Engineering, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, P. R. China.
Department Electrical Engineering, City University of Hong Kong, 83 Tat Chee Ave, Kowloon Tong, Hong Kong SAR, 999077, P. R. China.
Adv Mater. 2025 Jan;37(4):e2405847. doi: 10.1002/adma.202405847. Epub 2024 Dec 4.
Photodynamic therapy (PDT) is attracting widespread attention as a promising strategy for tumor treatment. However, the efficacy of PDT is severely limited by the insufficient tissue penetration depth of the light source and low reactive oxygen species (ROS) generation efficiency. Herein, the metal doping strategy is reported to construct a series of defect-rich M-doped amorphous CoMo-layered double hydroxide (a-M-CoMo-LDH, M = Mn, Cu, Al, Ni, Mg, Zn) photosensitizers (PSs) for NIR-II PDT. Especially, M-doped CoMo-LDH nanosheets are synthesized through a simple hydrothermal method and then etched by acid treatment to prepare defect-rich a-M-CoMo-LDH nanosheets. Under NIR-II 1270 nm laser irradiation, the defect-rich a-Zn-CoMo-LDH nanosheets exhibit the optimal PDT performance compared with other a-M-CoMo-LDH nanosheets, and also possess much higher ROS production activity (3.9 times) than that of the pristine a-CoMo-LDH, with a singlet oxygen quantum yield up to 1.86, which is the highest among all the reported PSs. After polyethylene glycol (PEG) modification, the a-Zn-CoMo-LDH-PEG nanosheets can function as an effective inorganic PS for PDT, effectively inducing cell apoptosis in vitro and eradicating tumors in vivo. Notably, transcriptome sequencing analysis and further molecular validation highlight the critical role of the apoptotic/p53/AMPK/oxidative phosphorylation signaling pathways in a-Zn-CoMo-LDH-PEG-induced cancer cell apoptosis.
光动力疗法（PDT）作为一种有前景的肿瘤治疗策略正受到广泛关注。然而，PDT的疗效受到光源组织穿透深度不足和活性氧（ROS）生成效率低的严重限制。在此，报道了一种金属掺杂策略，用于构建一系列富含缺陷的M掺杂非晶态钴钼层状双氢氧化物（a-M-CoMo-LDH，M = Mn、Cu、Al、Ni、Mg、Zn）用于近红外二区（NIR-II）PDT的光敏剂（PSs）。特别是，通过简单的水热法合成M掺杂的CoMo-LDH纳米片，然后通过酸处理进行蚀刻，以制备富含缺陷的a-M-CoMo-LDH纳米片。在近红外二区1270 nm激光照射下，与其他a-M-CoMo-LDH纳米片相比，富含缺陷的a-Zn-CoMo-LDH纳米片表现出最佳的PDT性能，并且其ROS产生活性也比原始的a-CoMo-LDH高得多（3.9倍），单线态氧量子产率高达1.86，这在所有报道的PSs中是最高的。经过聚乙二醇（PEG）修饰后，a-Zn-CoMo-LDH-PEG纳米片可作为一种有效的无机PS用于PDT，在体外有效诱导细胞凋亡并在体内根除肿瘤。值得注意的是，转录组测序分析和进一步的分子验证突出了凋亡/p53/AMPK/氧化磷酸化信号通路在a-Zn-CoMo-LDH-PEG诱导的癌细胞凋亡中的关键作用。
