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从人诱导多能干细胞（hiPSC）高效衍生功能性星形胶质细胞。

Efficient derivation of functional astrocytes from human induced pluripotent stem cells (hiPSCs).

作者信息

Szeky Balazs, Jurakova Veronika, Fouskova Eliska, Feher Anita, Zana Melinda, Karl Vivien Reka, Farkas Janos, Bodi-Jakus Maria, Zapletalova Martina, Pandey Shashank, Kucera Radek, Lochman Jan, Dinnyes Andras

机构信息

BioTalentum Ltd, Godollo, Hungary.

Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic.

出版信息

PLoS One. 2024 Dec 4;19(12):e0313514. doi: 10.1371/journal.pone.0313514. eCollection 2024.

DOI:10.1371/journal.pone.0313514
PMID:39630626
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11616838/
Abstract

Astrocytes are specialized glial cell types of the central nervous system (CNS) with remarkably high abundance, morphological and functional diversity. Astrocytes maintain neural metabolic support, synapse regulation, blood-brain barrier integrity and immunological homeostasis through intricate interactions with other cells, including neurons, microglia, pericytes and lymphocytes. Due to their extensive intercellular crosstalks, astrocytes are also implicated in the pathogenesis of CNS disorders, such as ALS (amyotrophic lateral sclerosis), Parkinson's disease and Alzheimer's disease. Despite the critical importance of astrocytes in neurodegeneration and neuroinflammation are recognized, the lack of suitable in vitro systems limits their availability for modeling human brain pathologies. Here, we report the time-efficient, reproducible generation of astrocytes from human induced pluripotent stem cells (hiPSCs). Our hiPSC-derived astrocytes expressed characteristic astrocyte markers, such as GFAP, S100b, ALDH1L1 and AQP4. Furthermore, hiPSC-derived astrocytes displayed spontaneous calcium transients and responded to inflammatory stimuli by the secretion of type A1 and type A2 astrocyte-related cytokines.

摘要

星形胶质细胞是中枢神经系统（CNS）中特殊类型的神经胶质细胞，数量极为丰富，具有形态和功能多样性。星形胶质细胞通过与包括神经元、小胶质细胞、周细胞和淋巴细胞在内的其他细胞进行复杂的相互作用，维持神经代谢支持、突触调节、血脑屏障完整性和免疫稳态。由于其广泛的细胞间串扰，星形胶质细胞也与中枢神经系统疾病的发病机制有关，如肌萎缩侧索硬化症（ALS）、帕金森病和阿尔茨海默病。尽管星形胶质细胞在神经退行性变和神经炎症中的关键重要性已得到认可，但缺乏合适的体外系统限制了它们用于模拟人类脑部疾病的可用性。在此，我们报告了从人诱导多能干细胞（hiPSC）中高效、可重复地生成星形胶质细胞的方法。我们的hiPSC来源的星形胶质细胞表达特征性的星形胶质细胞标志物，如GFAP、S100b、ALDH1L1和AQP4。此外，hiPSC来源的星形胶质细胞表现出自发性钙瞬变，并通过分泌A1型和A2型星形胶质细胞相关细胞因子对炎症刺激作出反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/555bd7191875/pone.0313514.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/c62cb985bd35/pone.0313514.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/ef76e656c064/pone.0313514.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/f3bc77681f47/pone.0313514.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/1ae96e09973f/pone.0313514.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/555bd7191875/pone.0313514.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/c62cb985bd35/pone.0313514.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/ef76e656c064/pone.0313514.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/f3bc77681f47/pone.0313514.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/1ae96e09973f/pone.0313514.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3450/11616838/555bd7191875/pone.0313514.g005.jpg

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J Neuroinflammation. 2024 Jan 4;21(1):7. doi: 10.1186/s12974-023-02951-2.
3
Comparison of two protocols for the generation of iPSC-derived human astrocytes.两种用于诱导多能干细胞（iPSC）来源的人星形胶质细胞生成方案的比较。
Biol Proced Online. 2023 Sep 20;25(1):26. doi: 10.1186/s12575-023-00218-x.
4
Specialized astrocytes mediate glutamatergic gliotransmission in the CNS.特化星形胶质细胞介导中枢神经系统中的谷氨酸能神经胶质传递。
Nature. 2023 Oct;622(7981):120-129. doi: 10.1038/s41586-023-06502-w. Epub 2023 Sep 6.
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