Carneiro Bárbara Gressy Duarte Souza, Maia Thays Allane Cordeiro, Costa Vanessa, Frieddrichsdorf Simone Peixe, Gondim Delane Viana, Pereira Karuza Maria Alves, Bezerra Mirna Marques, Goes Paula
Postgraduate program in Dentistry, Faculty of Pharmacy, Dentistry and Nursing, Federal University of Ceará, Fortaleza, CE, Brazil.
Postgraduate program in Translational Medicine, Faculty of Medicine, Federal University of Ceará, Fortaleza, CE, Brazil.
J Stomatol Oral Maxillofac Surg. 2025 Sep;126(4S):102175. doi: 10.1016/j.jormas.2024.102175. Epub 2024 Dec 2.
Reconstruction of bone defects prior to implant installation is a challenge, especially when the patient uses bisphosphonates. Given this difficulty, many studies investigate biomaterials that can improve the bone regeneration process. In this context, this study aimed to investigate the effect of platelet-rich fibrin (PRF) and Bio-Oss (BO) on bone regeneration of rats submitted to critical-sized calvaria defects and treated with ZA.
Thirty Wistar rats received a single dose of ZA (120 μg/kg) and after 7 days, were submitted to an 8 mm calvaria defect. The animals were divided into 5 groups (n = 6): ZA, BO, PRF or BO+PRF; animals from control group did not receive ZA. All animals were euthanized 12 weeks after surgical procedure and calvaria collected to histological, histomorphometric and micro-CT analyses.
BO+PRF increased the number of osteoblasts (33 %) and osteoclasts (58 %), as well as blood vessels (70 %) and Type I collagen (52 %) (p < 0.05) compared to ZA group.
In summary, the association of BO+PRF improved bone healing of large bone defect in rats receiving ZA and this may be an interesting approach for the treatment to be tested in patients under anti-resorptive therapy.
在植入种植体之前重建骨缺损是一项挑战，尤其是当患者使用双膦酸盐时。鉴于此困难，许多研究调查了可改善骨再生过程的生物材料。在此背景下，本研究旨在调查富血小板纤维蛋白（PRF）和Bio-Oss（BO）对接受唑来膦酸（ZA）治疗的大鼠临界大小颅骨缺损骨再生的影响。
30只Wistar大鼠接受单剂量的ZA（120μg/kg），7天后，制作8mm的颅骨缺损。动物分为5组（n = 6）：ZA组、BO组、PRF组或BO+PRF组；对照组动物未接受ZA。所有动物在手术12周后安乐死，收集颅骨进行组织学、组织形态计量学和显微CT分析。
与ZA组相比，BO+PRF组的成骨细胞数量增加了33%，破骨细胞数量增加了58%，血管数量增加了70%，I型胶原蛋白增加了52%（p < 0.05）。
总之，BO+PRF联合使用改善了接受ZA治疗的大鼠大骨缺损的骨愈合，这可能是一种值得在抗吸收治疗患者中进行测试的有趣治疗方法。
