Kelson Victoria, Kiser Jennifer, Davenport Kimberly, Suarez Emaly, Murdoch Brenda, Neibergs Holly
Washington State University Department of Animal Sciences, Pullman, Washington 99164, USA.
Washington State University Department of Animal Sciences, Pullman, Washington 99164, USA; Washington Animal Disease Diagnostic Laboratory, College of Veterinary Medicine, Pullman, Washington 99164, USA.
Genomics. 2025 Jan;117(1):110972. doi: 10.1016/j.ygeno.2024.110972. Epub 2024 Dec 2.
This study aimed to identify loci (p < 1 × 10) and gene sets (normalized enrichment score (NES) ≥ 3.0) associated with the number of times a heifer is bred to attain a successful pregnancy (TBRD) for Holstein heifers bred by artificial insemination (AI, n = 2754) or that were embryo transfer (ET, n = 1566) recipients. Eight loci were associated (p < 1 × 10) with TBRD in AI bred heifers and four loci were associated with TBRD in ET recipients. The gene set enrichment analysis with SNP data identified one gene set enriched (NES ≥ 3.0) with TBRD in AI bred heifers and two gene sets that were enriched with TBRD in ET recipients. The estimated pseudo-heritability for times bred to AI was 0.063 and 0.043 for ET. The identification of loci associated with embryonic loss aids in the selection of Holstein heifers with higher reproductive efficiencies that are AI bred or that are ET recipients.
本研究旨在确定与人工授精（AI，n = 2754）或胚胎移植（ET，n = 1566）受体的荷斯坦小母牛成功受孕所需配种次数（TBRD）相关的基因座（p < 1×10）和基因集（标准化富集分数（NES）≥ 3.0）。在人工授精的小母牛中，有8个基因座与TBRD相关（p < 1×10），在胚胎移植受体中有4个基因座与TBRD相关。利用SNP数据进行的基因集富集分析在人工授精的小母牛中鉴定出一个与TBRD富集相关（NES≥ 3.0）的基因集，在胚胎移植受体中有两个与TBRD富集相关的基因集。人工授精配种次数的估计假遗传力为0.063，胚胎移植为0.043。鉴定与胚胎损失相关的基因座有助于选择人工授精或胚胎移植受体中繁殖效率更高的荷斯坦小母牛。
