Department of Animal Sciences and Center for Reproductive Biology, Washington State University, Pullman, WA 99164, USA.
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.
Genes (Basel). 2020 Jul 8;11(7):767. doi: 10.3390/genes11070767.
Heifer conception rate (HCR) is defined as the percentage of inseminated heifers that become pregnant at each service. The genome-wide association analyses in this study focused on identifying the loci associated with Holstein heifer ( = 2013) conception rate at first service (HCR1) and the number of times bred (TBRD) to achieve a pregnancy. There were 348 unique loci associated ( < 5 × 10) with HCR1 and 615 unique loci associated ( < 5 × 10) with TBRD. The two phenotypes shared 302 loci, and 56 loci were validated in independent cattle populations. There were 52 transcription factor binding sites (TFBS) and 552 positional candidate genes identified in the HCR1- and TBRD-associated loci. The positional candidate genes and the TFBS associated with HCR1 and TBRD were used in the ingenuity pathway analysis (IPA). In the IPA, 11 pathways, 207 master regulators and 11 upstream regulators were associated ( < 1.23 × 10) with HCR1 and TBRD. The validated loci associated with both HCR1 and TBRD make good candidates for genomic selection and further investigations to elucidate the mechanisms associated with subfertility and infertility.
受胎率是指每一次输精中怀孕的后备牛的百分比。本研究的全基因组关联分析集中于鉴定与荷斯坦后备牛（n=2013）第一次输精受胎率（HCR1）和达到妊娠所需配种次数（TBRD）相关的基因座。与 HCR1 相关的独特基因座有 348 个（<5×10），与 TBRD 相关的独特基因座有 615 个（<5×10）。两个表型共有 302 个基因座，其中 56 个基因座在独立牛群中得到验证。在 HCR1 和 TBRD 相关基因座中鉴定到 52 个转录因子结合位点（TFBS）和 552 个定位候选基因。HCR1 和 TBRD 相关的定位候选基因和 TFBS 用于 Ingenuity 通路分析（IPA）。在 IPA 中，有 11 条通路、207 个主要调控因子和 11 个上游调控因子与 HCR1 和 TBRD 相关（<1.23×10）。与 HCR1 和 TBRD 均相关的验证基因座是基因组选择和进一步阐明与生育力低下和不孕相关机制的良好候选基因。
