Fakir Saikat, Sigdel Madan, Sarker Md Matiur Rahman, Folahan Joy T, Barabutis Nektarios
School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USA.
School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USA.
Cell Stress Chaperones. 2025 Feb;30(1):1-8. doi: 10.1016/j.cstres.2024.12.001. Epub 2024 Dec 2.
Endothelial injury can be the cause and consequence of severe inflammation and injury. Synthetic somatostatin analogs-which suppress Growth Hormone-are clinically-approved drugs associated with anti-inflammatory activities. In the present study, we suggest that the protective activities of Octreotide in human and bovine endothelial cells are mitigated by Ceapin-A7, which is an activating transcription factor 6 inhibitor. To study endothelial function, we assessed protein expression levels of key cytoskeletal proteins, as well as paracellular permeability. To evaluate inflammation, we measured factors that promote vascular leak, as well as reactive oxygen species generation. Collectively, our study supports the involvement of activating transcription factor 6 in the protective effects of Octreotide in endothelial barrier function.
内皮损伤可能是严重炎症和损伤的原因及后果。合成生长抑素类似物——可抑制生长激素——是临床上已获批的具有抗炎活性的药物。在本研究中，我们表明，Ceapin - A7（一种激活转录因子6抑制剂）可减弱奥曲肽对人及牛内皮细胞的保护作用。为研究内皮功能，我们评估了关键细胞骨架蛋白的蛋白表达水平以及细胞旁通透性。为评估炎症反应，我们测量了促进血管渗漏的因子以及活性氧的生成。总体而言，我们的研究支持激活转录因子6参与奥曲肽对内皮屏障功能的保护作用。
