Hanley F L, Grattan M T, Stevens M B, Hoffman J I
Am J Physiol. 1986 Apr;250(4 Pt 2):H558-66. doi: 10.1152/ajpheart.1986.250.4.H558.
The role of cardiac interstitial adenosine as an important metabolite in coronary autoregulation has not been established. We therefore measured steady-state cardiac interstitial adenosine concentration at a high and a low coronary inflow pressure using an epicardial diffusion well in anesthetized dogs. Although coronary resistance for the high and low pressure points showed highly significant differences (P less than 0.001), adenosine averaged 302 +/- 98 and 286 +/- 91 (SD) pmol/ml for the high and low pressure points, respectively (P greater than 0.20). Cardiac interstitial adenosine concentration was then measured with and without an intracoronary infusion of adenosine deaminase catalytic subunit. Adenosine averaged 28 +/- 21 (SD) pmol/ml during the infusion compared with 281 +/- 68 during control conditions (P less than 0.001). Finally, pressure-flow relations were obtained with and without the adenosine deaminase infusion, and there was no loss of autoregulation in the pressure of adenosine deaminase. These findings indicate that intracoronary adenosine deaminase markedly reduces interstitial adenosine concentration, that cardiac interstitial adenosine concentration remains constant during autoregulation, and that the coronary bed autoregulates normally when interstitial adenosine is reduced to levels close to zero. We conclude that cardiac interstitial adenosine concentration is not an important component in coronary autoregulation.
心脏间质腺苷作为冠状动脉自身调节中一种重要代谢产物的作用尚未明确。因此，我们使用心外膜扩散池在麻醉犬身上测量了高、低冠状动脉流入压下的稳态心脏间质腺苷浓度。尽管高、低压点的冠状动脉阻力显示出高度显著差异（P小于0.001），但高压点和低压点的腺苷平均浓度分别为302±98和286±91（标准差）pmol/ml（P大于0.20）。然后在冠状动脉内输注腺苷脱氨酶催化亚基前后测量心脏间质腺苷浓度。输注期间腺苷平均浓度为28±21（标准差）pmol/ml，而对照条件下为281±68（P小于0.001）。最后，在输注腺苷脱氨酶前后获得压力-流量关系，且腺苷脱氨酶作用下压力的自身调节未丧失。这些发现表明，冠状动脉内腺苷脱氨酶显著降低间质腺苷浓度，心脏间质腺苷浓度在自身调节过程中保持恒定，并且当间质腺苷降至接近零的水平时冠状动脉床仍能正常进行自身调节。我们得出结论，心脏间质腺苷浓度不是冠状动脉自身调节的重要组成部分。
