Allen R D, Stevenson K L, Roberts T K
Am J Reprod Immunol Microbiol. 1986 Jan;10(1):26-31. doi: 10.1111/j.1600-0897.1986.tb00005.x.
Conventional techniques used for the analysis of antisperm antibodies are not suited to the mouse model because of their requirements for relatively large amounts of serum and their inability to handle large numbers of samples. This has inhibited use of the mouse as an experimental model in areas involving antisperm immunity. As the ELISA technique has been successfully applied to analysis of antisperm antibody in human sera, we investigated its use as an assay for screening antisperm antibody in mouse serum. This report describes a simplified version of the ELISA technique that we have found to be successful for this purpose. The assay described can assess levels and classes of antisperm antibody in mouse serum and can also be used as a screening assay for monoclonal antibodies to mouse sperm. It should facilitate use of the mouse in experimental work in areas involving assessment of immunity to sperm.
用于分析抗精子抗体的传统技术不适合小鼠模型，因为它们需要相对大量的血清，并且无法处理大量样本。这限制了在涉及抗精子免疫的领域中使用小鼠作为实验模型。由于酶联免疫吸附测定（ELISA）技术已成功应用于分析人血清中的抗精子抗体，我们研究了将其用作筛选小鼠血清中抗精子抗体的检测方法。本报告描述了一种简化版的ELISA技术，我们发现该技术在此方面很成功。所描述的检测方法可以评估小鼠血清中抗精子抗体的水平和类别，也可用作筛选抗小鼠精子单克隆抗体的检测方法。它应有助于在涉及评估精子免疫的领域中使用小鼠进行实验工作。
