Luo Yuan, Tseng Ming Lun, Vyas Sunil, Hsieh Ting-Yu, Wu Jui-Ching, Chen Shang-Yang, Peng Hsiao-Fang, Su Vin-Cent, Huang Tzu-Ting, Kuo Hsin Yu, Chu Cheng Hung, Chen Mu Ku, Chen Jia-Wern, Chen Yu-Chun, Huang Kuang-Yuh, Kuan Chieh-Hsiung, Shi Xu, Misawa Hiroaki, Tsai Din Ping
National Taiwan University, Institute of Medical Device and Imaging, No. 1 Ren Ai Rd. Sect. 1, Taipei, 10051, Taiwan, ROC.
Institute of Electronics, National Yang Ming Chiao Tung University, Hsinchu, Taiwan, ROC.
Nanophotonics. 2022 Feb 21;11(9):1949-1959. doi: 10.1515/nanoph-2021-0748. eCollection 2022 Apr.
Light-sheet fluorescent microscopy has become the leading technique for imaging in the fields of disease, medicine, and cell biology research. However, designing proper illumination for high image resolution and optical sectioning is challenging. Another issue is geometric constraints arising from the multiple bulky components for illumination and detection. Here, we demonstrate that those issues can be well addressed by integrating nanophotonic meta-lens as the illumination component for LSFM. The meta-lens is composed of 800-nm-thick GaN nanostructures and is designed for a light-sheet well-adapted to biological specimens such as the nematode (). With the meta-lens, the complexity of the LSFM system is significantly reduced, and it is capable of performing multicolor fluorescent imaging of live with cellular resolution. Considering the miniature size and plane geometry of the meta-lens, our system enables a new design for LSFM to acquire images of biological specimens with high resolution.
光片荧光显微镜已成为疾病、医学和细胞生物学研究领域成像的领先技术。然而，设计用于高图像分辨率和光学切片的适当照明具有挑战性。另一个问题是由用于照明和检测的多个大型组件产生的几何约束。在这里，我们证明通过集成纳米光子超透镜作为光片荧光显微镜（LSFM）的照明组件，可以很好地解决这些问题。该超透镜由800纳米厚的氮化镓纳米结构组成，设计用于与线虫等生物样本良好适配的光片。有了该超透镜，光片荧光显微镜系统的复杂性显著降低，并且能够以细胞分辨率对活样本进行多色荧光成像。考虑到超透镜的微型尺寸和平面几何形状，我们的系统为光片荧光显微镜提供了一种新的设计，能够以高分辨率获取生物样本的图像。
