Xie Qi, Wang Tianqi, He Lina, Liang Hongbo, Sun Jingxuan, Huang Xiaoxiao, Xie Weili, Niu Yumei
The First Affiliated Hospital of Harbin Medical University, School of Stomatology, Harbin Medical University, Harbin, China.
School of Materials Science and Engineering, Harbin Institute of Technology, Harbin, China.
Front Bioeng Biotechnol. 2024 Nov 20;12:1505102. doi: 10.3389/fbioe.2024.1505102. eCollection 2024.
To address the challenges related to bone defects, including osteoinductivity deficiency and post-implantation infection risk, this study developed the collagen composite scaffolds (CUR-GO-COL) with multifunctionality by integrating the curcumin-loaded graphene oxide with collagen through a freeze-drying-cross-linking process.
The morphological and structural characteristics of the composite scaffolds were analyzed, along with their physicochemical properties, including water absorption capacity, water retention rate, porosity, degradation, and curcumin release. To evaluate the biocompatibility, cell viability, proliferation, and adhesion capabilities of the composite scaffolds, as well as their osteogenic and antimicrobial properties, in vitro cell and bacterial assays were conducted. These assays were designed to assess the impact of the composite scaffolds on cell behavior and bacterial growth, thereby providing insights into their potential for promoting osteogenesis and inhibiting infection.
The CUR-GO-COL composite scaffold with a CUR-GO concentration of 0.05% (w/v) exhibits optimal biological compatibility and stable and slow curcumin release rate. Furthermore, cell and bacterial tests demonstrated that the prepared CUR-GO-COL composite scaffolds enhance cell viability, proliferation and adhesion, and offer superior osteogenic and antimicrobial properties compared with the CUR-GO composite scaffold, confirming the osteogenesis promotion and antimicrobial effects.
The introduction of CUR-GO into collagen scaffold creates a bone-friendly microenvironment, and offers a theoretical foundation for the design, investigation and utilization of multifunctional bone tissue biomaterials.
为应对与骨缺损相关的挑战，包括骨诱导性不足和植入后感染风险，本研究通过冷冻干燥交联工艺将负载姜黄素的氧化石墨烯与胶原蛋白整合，开发出具有多功能性的胶原蛋白复合支架（CUR-GO-COL）。
分析了复合支架的形态和结构特征，以及它们的物理化学性质，包括吸水能力、保水率、孔隙率、降解和姜黄素释放。为评估复合支架的生物相容性、细胞活力、增殖和粘附能力，以及它们的成骨和抗菌性能，进行了体外细胞和细菌试验。这些试验旨在评估复合支架对细胞行为和细菌生长的影响，从而深入了解其促进成骨和抑制感染的潜力。
CUR-GO浓度为0.05%（w/v）的CUR-GO-COL复合支架表现出最佳的生物相容性以及稳定且缓慢的姜黄素释放率。此外，细胞和细菌测试表明，与CUR-GO复合支架相比，所制备的CUR-GO-COL复合支架可提高细胞活力、增殖和粘附能力，并具有优异的成骨和抗菌性能，证实了其促进成骨和抗菌作用。
将CUR-GO引入胶原蛋白支架中创造了一个有利于骨生长的微环境，并为多功能骨组织生物材料的设计、研究和应用提供了理论基础。
