Lee Kangwook, Pan Jeong Hoon, Choi La Yoon, Ju Jaehyun, Le Brandy, Williams Liana C, Cho Tae Jin, Lee Eunjin, Yoon Ji Soo, Park Chae Lee, Kim Sang-Yoon, Yeon Sung Hum, Kim Jeonghoon, Choi Mulim, Kim Kongsik, Kim Kee-Hong, Kim Jae Kyeom
Department of Food and Biotechnology, Korea University, Sejong, Republic of Korea.
Department of Food Science and Nutrition, and The Basic Science Institute of Chosun University, Chosun University, Gwangju, Republic of Korea.
Front Nutr. 2024 Nov 20;11:1507886. doi: 10.3389/fnut.2024.1507886. eCollection 2024.
Post-harvest processing plays a crucial role in enhancing the bioactive properties of vegetables. This study aimed to investigate the impact of post-harvest aging on parsnip's bioactive profile and its protective effects against acrolein (Acr)-induced inflammation, a common pollutant and irritant linked to respiratory inflammation.
Parsnips ( L.) were aged at 60°C for up to 30 days, with extracts collected at intervals. Total phenolic content (TPC) and antioxidant capacity were assessed using DPPH assays. Key bioactive compounds, including falcarindiol, DDMP, and 5-HMF, were quantified. In vitro studies used BEAS-2B cells to evaluate anti-inflammatory effects, while tests involved treating Acr-exposed mice with aged parsnip extract to observe cytokine responses.
Aged parsnip extracts showed a 9.96-fold increase in TPC and a 4.25-fold increase in antioxidant capacity after 30 days. Bioactive compounds significantly increased in aged samples, especially falcarindiol and 5-HMF. , aged parsnip reduced Acr-induced TNF-α and IL-1β expression. , treated mice showed reduced bronchial inflammation, goblet cell hyperplasia, and cytokine expression compared to controls.
These findings suggest that post-harvest aging enhances parsnip's antioxidant and anti-inflammatory properties, highlighting its potential as a functional food ingredient for managing inflammation and respiratory health.
采后处理在增强蔬菜的生物活性特性方面起着至关重要的作用。本研究旨在调查采后老化对防风草生物活性特征的影响及其对丙烯醛（Acr）诱导的炎症的保护作用，丙烯醛是一种与呼吸道炎症相关的常见污染物和刺激物。
将防风草在60°C下老化长达30天，定期收集提取物。使用DPPH测定法评估总酚含量（TPC）和抗氧化能力。对包括镰叶芹二醇、DDMP和5-羟甲基糠醛在内的关键生物活性化合物进行定量。体外研究使用BEAS-2B细胞评估抗炎作用，而体内试验则用老化的防风草提取物处理暴露于Acr的小鼠，以观察细胞因子反应。
老化30天后，防风草提取物的TPC增加了9.96倍，抗氧化能力增加了4.25倍。老化样品中的生物活性化合物显著增加，尤其是镰叶芹二醇和5-羟甲基糠醛。体外，老化的防风草降低了Acr诱导的TNF-α和IL-1β表达。体内，与对照组相比，经处理的小鼠支气管炎症、杯状细胞增生和细胞因子表达减少。
这些发现表明，采后老化增强了防风草的抗氧化和抗炎特性，突出了其作为管理炎症和呼吸道健康的功能性食品成分的潜力。
