• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

红细胞信号传导在侵袭过程中功能保守。

Red blood cell signaling is functionally conserved in invasion.

作者信息

Yong James Jia Ming, Gao Xiaohong, Prakash Prem, Ang Jing Wen, Lai Soak Kuan, Chen Ming Wei, Neo Jason Jun Long, Lescar Julien, Li Hoi Yeung, Preiser Peter R

机构信息

School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, Singapore.

出版信息

iScience. 2024 Sep 26;27(10):111052. doi: 10.1016/j.isci.2024.111052. eCollection 2024 Oct 18.

DOI:10.1016/j.isci.2024.111052
PMID:39635131
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11615254/
Abstract

It is widely recognized that merozoites secrete ligands that interact with RBC receptors. Meanwhile the question on whether these interactions trigger RBC signals essential for invasion remains unresolved. There is evidence that parasites manipulate native RBC Ca signaling to facilitate invasion. Here, we demonstrate a key role of RBC Ca influx that is conserved across different species during invasion. RH5-basigin interaction triggers RBC cAMP increase to promote Ca influx. The RBC signaling pathways can be blocked by a range of inhibitors during invasion, providing the evidence of a functionally conserved host cAMP-Ca signaling that drives invasion and junction formation. Furthermore, RH5-basigin binding induces a pre-existing multimeric RBC membrane complex to undergo increased protein association containing the cAMP-inducing β-adrenergic receptor. Our work presents evidence of a conserved host cell signaling cascade necessary for invasion and will create opportunities to therapeutically target merozoite invasion.

摘要

普遍认为，裂殖子分泌与红细胞（RBC）受体相互作用的配体。与此同时，这些相互作用是否触发入侵所必需的红细胞信号这一问题仍未得到解决。有证据表明，寄生虫操纵天然红细胞的钙信号以促进入侵。在此，我们证明了红细胞钙内流在不同物种入侵过程中的关键作用，该作用具有保守性。RH5-基底结合素相互作用触发红细胞环磷酸腺苷（cAMP）增加以促进钙内流。在入侵过程中，一系列抑制剂可阻断红细胞信号通路，这为驱动入侵和连接形成的功能保守的宿主cAMP-钙信号提供了证据。此外，RH5-基底结合素结合诱导预先存在的多聚红细胞膜复合物增加蛋白质结合，其中包含诱导cAMP的β-肾上腺素能受体。我们的工作为入侵所必需的保守宿主细胞信号级联提供了证据，并将为治疗性靶向裂殖子入侵创造机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/3ae4d668d76c/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/c57d4ab3fe13/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/e2544165dbef/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/19a2f435d1ef/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/12912c7ad828/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/09325db4a424/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/5fb522fd8729/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/c6062db0266d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/b1856179b567/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/3ae4d668d76c/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/c57d4ab3fe13/fx1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/e2544165dbef/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/19a2f435d1ef/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/12912c7ad828/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/09325db4a424/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/5fb522fd8729/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/c6062db0266d/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/b1856179b567/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f093/11615254/3ae4d668d76c/gr8.jpg

相似文献

1
Red blood cell signaling is functionally conserved in invasion.红细胞信号传导在侵袭过程中功能保守。
iScience. 2024 Sep 26;27(10):111052. doi: 10.1016/j.isci.2024.111052. eCollection 2024 Oct 18.
2
P. falciparum RH5-Basigin interaction induces changes in the cytoskeleton of the host RBC.恶性疟原虫 RH5-Basigin 相互作用诱导宿主 RBC 细胞骨架发生变化。
Cell Microbiol. 2017 Sep;19(9). doi: 10.1111/cmi.12747. Epub 2017 May 9.
3
Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites.磷酸化依赖性的 14-3-3 介导的信号复合物在恶性疟原虫裂殖子入侵红细胞中的组装。
mBio. 2020 Aug 18;11(4):e01287-20. doi: 10.1128/mBio.01287-20.
4
RBC membrane biomechanics and Plasmodium falciparum invasion: probing beyond ligand-receptor interactions.红细胞膜生物力学与恶性疟原虫入侵：超越配体-受体相互作用的探索。
Trends Parasitol. 2022 Apr;38(4):302-315. doi: 10.1016/j.pt.2021.12.005. Epub 2022 Jan 4.
5
Host-parasite interactions that guide red blood cell invasion by malaria parasites.指导疟原虫入侵红细胞的宿主-寄生虫相互作用。
Curr Opin Hematol. 2015 May;22(3):220-6. doi: 10.1097/MOH.0000000000000135.
6
The dual action of human antibodies specific to Plasmodium falciparum PfRH5 and PfCyRPA: Blocking invasion and inactivating extracellular merozoites.人源抗疟原虫 PfRH5 和 PfCyRPA 抗体的双重作用：阻断入侵和使外质子虫无效化。
PLoS Pathog. 2023 Sep 15;19(9):e1011182. doi: 10.1371/journal.ppat.1011182. eCollection 2023 Sep.
7
Adaptive Evolution of RH5 in Ape species of the Subgenus.RH5 在亚属人猿物种中的适应性进化。
mBio. 2018 Jan 23;9(1):e02237-17. doi: 10.1128/mBio.02237-17.
8
Morphology and kinetics of the three distinct phases of red blood cell invasion by Plasmodium falciparum merozoites.恶性疟原虫裂殖子入侵红细胞三个不同阶段的形态学与动力学
Int J Parasitol. 2009 Jan;39(1):91-6. doi: 10.1016/j.ijpara.2008.09.007. Epub 2008 Oct 11.
9
Dynamic Palmitoylation of Red Cell Membrane Proteins Governs Susceptibility to Invasion by the Malaria Parasite, .红细胞膜蛋白的动态棕榈酰化调控疟疾寄生虫的入侵易感性。
ACS Infect Dis. 2022 Oct 14;8(10):2106-2118. doi: 10.1021/acsinfecdis.2c00199. Epub 2022 Aug 31.
10
PfRH2b specific monoclonal antibodies inhibit merozoite invasion.PfRH2b特异性单克隆抗体抑制裂殖子入侵。
Mol Microbiol. 2016 Nov;102(3):386-404. doi: 10.1111/mmi.13468. Epub 2016 Aug 18.

引用本文的文献

1
CD44 cross-linking promotes invasion.CD44交联促进侵袭。
bioRxiv. 2025 Jul 30:2025.07.30.667750. doi: 10.1101/2025.07.30.667750.
2
PfRH5 vaccine; from the bench to the vial.PfRH5疫苗：从实验室到药瓶
NPJ Vaccines. 2025 Apr 24;10(1):82. doi: 10.1038/s41541-025-01137-6.

本文引用的文献

1
Proteomic Analysis of the Role of the Adenylyl Cyclase-cAMP Pathway in Red Blood Cell Mechanical Responses.蛋白质组学分析腺苷酸环化酶-cAMP 通路在红细胞机械反应中的作用。
Cells. 2022 Apr 6;11(7):1250. doi: 10.3390/cells11071250.
2
Gliding motility of merozoites.裂殖子的滑行运动。
Proc Natl Acad Sci U S A. 2021 Nov 30;118(48). doi: 10.1073/pnas.2114442118.
3
Zoonotic Malaria: Non- Biology and Invasion Mechanisms.人畜共患疟疾：非生物学及入侵机制
Pathogens. 2021 Jul 13;10(7):889. doi: 10.3390/pathogens10070889.
4
Erythrocyte Binding Like Protein Interacts With Basigin, an Erythrocyte Surface Protein.红细胞结合样蛋白与红细胞表面蛋白基底素相互作用。
Front Cell Infect Microbiol. 2021 Apr 14;11:656620. doi: 10.3389/fcimb.2021.656620. eCollection 2021.
5
Basigin drives intracellular accumulation of l-lactate by harvesting protons and substrate anions.Basigin 通过收集质子和底物阴离子来驱动 l-乳酸的细胞内积累。
PLoS One. 2021 Mar 26;16(3):e0249110. doi: 10.1371/journal.pone.0249110. eCollection 2021.
6
Subcellular Organization of the cAMP Signaling Pathway.细胞信号转导途径 cAMP 的亚细胞结构。
Pharmacol Rev. 2021 Jan;73(1):278-309. doi: 10.1124/pharmrev.120.000086.
7
Characterization of the erythrocyte GTPase Rac1 in relation to Plasmodium falciparum invasion.红细胞 GTPase Rac1 的特性与恶性疟原虫入侵的关系。
Sci Rep. 2020 Dec 16;10(1):22054. doi: 10.1038/s41598-020-79052-0.
8
CD147-spike protein is a novel route for SARS-CoV-2 infection to host cells.CD147-刺突蛋白是 SARS-CoV-2 感染宿主细胞的新途径。
Signal Transduct Target Ther. 2020 Dec 4;5(1):283. doi: 10.1038/s41392-020-00426-x.
9
cAMP signalling and its role in host cell invasion by malaria parasites.cAMP 信号转导及其在疟原虫感染宿主细胞中的作用。
Curr Opin Microbiol. 2020 Dec;58:69-74. doi: 10.1016/j.mib.2020.09.003. Epub 2020 Oct 5.
10
Intracellular Ca Concentration and Phosphatidylserine Exposure in Healthy Human Erythrocytes in Dependence on Cell Age.健康人红细胞内钙离子浓度及磷脂酰丝氨酸暴露与细胞年龄的关系
Front Physiol. 2020 Jan 10;10:1629. doi: 10.3389/fphys.2019.01629. eCollection 2019.