Le Febvre R B, Thiermann A B
Am J Vet Res. 1986 Apr;47(4):959-63.
Hybridization studies of chromosomal DNA from leptospiral strains representing Leptospira interrogans, serogroups Sejroe and Pomona from cattle and swine were performed to determine the degree of homology among their DNA sequences. Chromosomal DNA isolated from leptospires of the Sejroe and Pomona serogroups was radiolabeled and used to probe DNA isolated from other strains in these serogroups. Serovars hardjo (hardjoprajitno), hardjo (hardjo-bovis), balcanica (1627 Burgas), pomona (pomona), and kennewicki (LT-1026) were probed to determine the degree of homology among their chromosomes. Serovars pomona and kennewicki were homologous to each other. They also had a high degree of homology with hardjo (hardjoprajitno) and, to a lesser extent, with hardjo (hardjo-bovis) strains. However, hardjoprajitno and hardjo-bovis had little homology to each other. Serovar balcanica had a high degree of homology with hardjo-bovis isolates, but little homology with hardjoprajitno. Although serologically indistinguishable, the reference strain hardjoprajitno was genetically dissimilar to hardjo-bovis strains isolated from North American cattle.
对来自代表问号钩端螺旋体、牛和猪的 sejroe 血清群和波摩那群的钩端螺旋体菌株的染色体 DNA 进行杂交研究，以确定它们 DNA 序列之间的同源程度。从 sejroe 和波摩那群钩端螺旋体中分离的染色体 DNA 被放射性标记，并用于探测从这些血清群中的其他菌株分离的 DNA。对哈德乔（哈德乔普拉吉特诺）、哈德乔（哈德乔-牛型）、巴尔干尼卡（1627 布尔加斯）、波摩那（波摩那）和肯纽维奇（LT-1026）血清型进行探测，以确定它们染色体之间的同源程度。波摩那和肯纽维奇血清型彼此同源。它们与哈德乔（哈德乔普拉吉特诺）也有高度同源性，与哈德乔（哈德乔-牛型）菌株的同源性程度较低。然而，哈德乔普拉吉特诺和哈德乔-牛型彼此之间同源性很小。巴尔干尼卡血清型与哈德乔-牛型分离株有高度同源性，但与哈德乔普拉吉特诺同源性很小。尽管在血清学上无法区分，但参考菌株哈德乔普拉吉特诺在基因上与从北美牛分离的哈德乔-牛型菌株不同。
