LeFebvre R B, Lane R S, Perng G C, Brown J A, Johnson R C
Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
J Clin Microbiol. 1990 Apr;28(4):700-7. doi: 10.1128/jcm.28.4.700-707.1990.
Nine isolates of Borrelia burgdorferi from ixodid ticks collected in northern California were characterized. Restriction endonuclease analysis, pulsed-field gel electrophoresis, and Western blot (immunoblot) analysis were used in this study. Four isolates were very similar to each other. The others shared some similarities but were classified as having unique genotypes. A strain from an Ixodes neotomae tick displayed the greatest genetic and antigenic diversity when compared to the isolates collected from Ixodes pacificus ticks. A computerized library based on DNA banding patterns of the isolates by restriction enzyme analysis is also reported. This library was created by using a scanning laser densitometer.
对从加利福尼亚北部采集的硬蜱中分离出的9株伯氏疏螺旋体进行了鉴定。本研究采用了限制性内切酶分析、脉冲场凝胶电泳和蛋白质免疫印迹（免疫印迹）分析。4株分离株彼此非常相似。其他分离株有一些相似之处，但被归类为具有独特的基因型。与从太平洋硬蜱分离出的菌株相比，一株来自新黑腹硬蜱的菌株表现出最大的遗传和抗原多样性。还报道了一个基于分离株DNA条带模式通过限制性酶切分析建立的计算机化文库。该文库是使用扫描激光密度计创建的。
