Abe T, Okamoto E, Itokawa Y
J Nutr Sci Vitaminol (Tokyo). 1979;25(5):375-83. doi: 10.3177/jnsv.25.375.
Rats were separated into four groups and four different liquid diets were given to each group. Group 1: thiamine-sufficient diet with no ethanol, group 2: thiamine-sufficient diet with ethanol, group 3: thiamine-deficient diet with no ethanol, group 4: thiamine-deficient diet with ethanol. After four weeks, all rats were fasted for 24 hr and then ethanol was given orally to every rat. After one hour, every rat was sacrificed and biochemical and histological analyses were carried out. Transketolase activity in brain and liver decreased in groups 2, 3 and 4. There was significant decrease in transketolase activity in ethanol-fed groups (groups 2 and 4) as compared to control groups (groups 1 and 3). Ethanol concentrations in blood, liver and heart of rats in groups 2 and 4 were higher than in groups 1 and 3. When comparison was made between the thiamine-deficient groups and the corresponding thiamine-sufficient groups, ethanol concentrations in liver and heart were higher in the thiamine-deficient groups. Alcohol dehydrogenase activity in liver decreased significantly in groups 2 and 4. By histological analyses, fatty degeneration was observed in the livers of groups 2 and 4. The degeneration was more prominent in group 4 than group 2. These findings suggest that chronic ethanol administration may impair the ability to metabolize ethanol and the impairment may increase when rats are in the condition of thiamine deficiency.
大鼠被分为四组，每组给予四种不同的液体饮食。第1组：含硫胺素充足且无乙醇的饮食；第2组：含硫胺素充足且有乙醇的饮食；第3组：硫胺素缺乏且无乙醇的饮食；第4组：硫胺素缺乏且有乙醇的饮食。四周后，所有大鼠禁食24小时，然后给每只大鼠口服乙醇。一小时后，处死每只大鼠并进行生化和组织学分析。第2、3和4组大鼠脑和肝脏中的转酮醇酶活性降低。与对照组（第1和3组）相比，乙醇喂养组（第2和4组）的转酮醇酶活性显著降低。第2和4组大鼠血液、肝脏和心脏中的乙醇浓度高于第1和3组。当硫胺素缺乏组与相应的硫胺素充足组进行比较时，硫胺素缺乏组肝脏和心脏中的乙醇浓度更高。第2和4组大鼠肝脏中的乙醇脱氢酶活性显著降低。通过组织学分析，在第2和4组大鼠的肝脏中观察到脂肪变性。第4组的变性比第2组更明显。这些发现表明，长期给予乙醇可能会损害乙醇代谢能力，并且当大鼠处于硫胺素缺乏状态时，这种损害可能会增加。
