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转录组比较揭示了不同饲养条件下青海牦牛皮下脂肪代谢的差异。

Transcriptome comparison revealed the difference in subcutaneous fat metabolism of Qinghai yak under different feeding conditions.

作者信息

Ding Weiqin, Sun Yonggang, Han Yincang, Liu Yaqian, Jin Shengwei

机构信息

Academy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, Qinghai, China.

Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization of Qinghai Provincial, Xining, Qinghai, China.

出版信息

PLoS One. 2024 Dec 5;19(12):e0311224. doi: 10.1371/journal.pone.0311224. eCollection 2024.

DOI:10.1371/journal.pone.0311224
PMID:39637129
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11620555/
Abstract

In order to explore the differences in subcutaneous fat metabolism and pathway information in yaks under different feeding conditions, this experiment used Illumina high-throughput sequencing technology to sequence the transcriptome of subcutaneous fat tissues of yaks under different feeding conditions and analyzed them bioinformatically. 9 naturally grazed yaks at 18 months of age were randomly divided into 3 groups, one group (G18_SF) was slaughtered, one group (G24_SF) continued to graze until 24 months of age was slaughtered, and one group (F24_SF) was housed until 24 months of age was slaughtered, and subcutaneous fat tissue was collected from the back of the yaks. A total of 15,261 expressed genes were identified in the nine samples, with 13,959 coexpressed genes and 533 differential expressed genes (DEGs), G18_SF vs F24_SF 133 DEGs, G18_SF vs G24_SF 469 DEGs, F24_SF vs G24_SF 5 DEGs. GO functional annotation analysis found that DEGs were mainly annotated in BP and CC, which included biological regulation, metabolic processes and cellular processes. KEGG revealed that the DEGs are mainly enriched for PPAR signaling pathway, AMPK signaling pathway and other pathways related to lipid metabolism. This study provides a scientific basis for further research on the effects of mRNA on subcutaneous fat in yaks under different feeding conditions.

摘要

为探究不同饲养条件下牦牛皮下脂肪代谢及通路信息的差异，本实验采用Illumina高通量测序技术对不同饲养条件下牦牛皮下脂肪组织转录组进行测序，并进行生物信息学分析。选取9头18月龄自然放牧的牦牛，随机分为3组，一组（G18_SF）屠宰，一组（G24_SF）继续放牧至24月龄屠宰，一组（F24_SF）舍饲至24月龄屠宰，采集牦牛背部皮下脂肪组织。9个样本共鉴定出15261个表达基因，其中共表达基因13959个，差异表达基因（DEG）533个，G18_SF与F24_SF比较有133个DEG，G18_SF与G24_SF比较有469个DEG，F24_SF与G24_SF比较有5个DEG。GO功能注释分析发现，DEG主要注释在BP和CC中，包括生物调控、代谢过程和细胞过程。KEGG分析表明，DEG主要富集在PPAR信号通路、AMPK信号通路等与脂质代谢相关的通路。本研究为进一步研究mRNA对不同饲养条件下牦牛皮下脂肪的影响提供了科学依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/6fa91f845657/pone.0311224.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/b1d3ab9f0ce5/pone.0311224.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/b47830e6c146/pone.0311224.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/759de239c77d/pone.0311224.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/8cf6119537f2/pone.0311224.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/6fa91f845657/pone.0311224.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/b1d3ab9f0ce5/pone.0311224.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/b47830e6c146/pone.0311224.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/759de239c77d/pone.0311224.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/8cf6119537f2/pone.0311224.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e144/11620555/6fa91f845657/pone.0311224.g005.jpg

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