Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, China.
Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, China.
Poult Sci. 2023 Mar;102(3):102429. doi: 10.1016/j.psj.2022.102429. Epub 2022 Dec 20.
This study aimed to investigate the effect of a high-fat diet on the growth performance, serum, liver, and skin lipid metabolism as well as the fatty acids composition of liver and skin fat in Pekin ducks from 10 to 40 d of age based on a pair-fed group. Two hundred forty healthy male ducks (10 d old, 470.53 ± 0.57 g) were randomly divided into 3 groups (8 replicates per cage of 10 ducks): a normal diet (ND, 3% fat), a high-fat diet (HFD, 9% fat), and a pair-fed diet (PFD, given the ND in an amount equal to that consumed of the HFD to eliminate the effects of feed intake). The results were as follows: compared to ND feeding, HFD feeding significantly decreased (P < 0.05) the feed intake and feed:gain ratio (F:G), along with serum triglyceride and nonesterified fatty acid contents. When compared with the ND and PFD, the HFD significantly decreased (P < 0.05) the liver weight and inhibited hepatic de novo lipogenesis (glucose-6-phosphate dehydrogenase and malate dehydrogenase activities), β-oxidation (carnitine palmitoyltransferase-1 content), and decreased saturated fatty acids and monounsaturated fatty acids deposition. Moreover, the HFD significantly increased (P < 0.05) the total fat content, lipid droplet area, and polyunsaturated fatty acids (PUFAs) content in the liver, as well as the abdominal fat weight, subcutaneous fat weight, the total fat and PUFAs content in skin fat. These results suggested that the HFD improved feed efficiency, which was related to HFD feeding inhibiting hepatic de novo lipogenesis and β-oxidation and promoting the deposition of fat in skin as well as altering the fatty acids composition of the liver and skin fat in Pekin ducks.
本研究旨在通过配对喂养组研究高脂肪饮食对 10 至 40 日龄北京鸭生长性能、血清、肝脏和皮肤脂质代谢以及肝脏和皮肤脂肪脂肪酸组成的影响。将 240 只健康雄性北京鸭（10 日龄，470.53±0.57g）随机分为 3 组（每笼 10 只，8 个重复）：正常日粮（ND，3%脂肪）、高脂肪日粮（HFD，9%脂肪）和配对喂养日粮（PFD，按 HFD 的摄入量给予 ND，以消除饲料摄入的影响）。结果表明：与 ND 相比，HFD 显著降低（P<0.05）了饲料摄入量和饲料增重比（F：G），同时血清甘油三酯和非酯化脂肪酸含量也降低。与 ND 和 PFD 相比，HFD 显著降低（P<0.05）了肝脏重量，并抑制了肝从头合成脂肪（葡萄糖-6-磷酸脱氢酶和苹果酸脱氢酶活性）、β-氧化（肉碱棕榈酰转移酶-1 含量），并降低了饱和脂肪酸和单不饱和脂肪酸的沉积。此外，HFD 还显著增加了（P<0.05）肝脏总脂肪含量、脂滴面积和多不饱和脂肪酸（PUFAs）含量，以及腹部脂肪重量、皮下脂肪重量、皮肤脂肪总脂肪和 PUFAs 含量。这些结果表明，HFD 提高了饲料效率，这与 HFD 抑制肝从头合成脂肪和β-氧化以及促进皮肤脂肪沉积和改变北京鸭肝脏和皮肤脂肪脂肪酸组成有关。
