Keim V
Ann Nutr Metab. 1986;30(2):113-9. doi: 10.1159/000177183.
The influence of fasting and dietary modulation on basal and stimulated pancreatic enzyme secretion was studied in conscious rats with cannulated pancreatic ducts. Two days' fasting decreased pancreatic secretion by about 30%. After stimulation of the secretion rate by acute derivation of pancreatic juice or by intravenous infusion of cerulein (0.2 micrograms/kg/h) no rapid change of enzyme composition was found. In further experiments, two groups of rats were fed with carbohydrate-rich and carbohydrate-poor diets, respectively. After pancreatic duct cannulation and a postsurgical recovery period, the diets were interchanged. An adaptation of pancreatic enzyme secretion to the new diet was observed in pancreatic juice. Stimulation of the secretion rate during the adaptation process by acute derivation of the juice or by intravenous infusion of either cerulein, secretin, carbachol or cerulein plus secretin did not further modify the composition of pancreatic proteins. 'Nonparallel' secretion of enzymes was never found when sufficient time for recovery from pancreatic duct surgery was allowed.
在有胰腺导管插管的清醒大鼠中，研究了禁食和饮食调节对基础及刺激状态下胰腺酶分泌的影响。禁食两天使胰腺分泌减少约30%。通过急性引出胰液或静脉输注雨蛙素（0.2微克/千克/小时）刺激分泌速率后，未发现酶成分有快速变化。在进一步的实验中，两组大鼠分别喂食富含碳水化合物和碳水化合物含量低的饮食。在进行胰腺导管插管及术后恢复期后，更换饮食。在胰液中观察到胰腺酶分泌适应了新饮食。在适应过程中，通过急性引出胰液或静脉输注雨蛙素、促胰液素、卡巴胆碱或雨蛙素加促胰液素刺激分泌速率，并未进一步改变胰腺蛋白质的成分。当给予足够时间从胰腺导管手术中恢复时，从未发现酶的“非平行”分泌。
