Sarfati P, Green G M, Morisset J
Department of Biology, Faculty of Sciences, Sherbrooke University, Quebec, Canada.
Pancreas. 1988;3(4):375-82. doi: 10.1097/00006676-198808000-00002.
These studies were undertaken to assess the effects of chronic administration of cerulein plus secretin, every 8 h for 10 days on fluid, protein, and somatostatin-like immunoreactivity (SLI) outputs in rat pancreatic juice. Pancreatic secretion was studied with conscious, cannulated rats, during infusion of precollected pancreatic juice to the rat intestine. Volume output reached a plateau of 5.2 ml/210 min after 3 days of treatment. Protein secretion steadily increased up to 218 mg/210 min after 5 days, decreased to 118 mg/210 min by day 7, and rose again to 281 mg/210 min after 10 days. The SLI release increased stepwise with a first plateau after 3 days at 493 ng/210 min; values between 983 and 562 ng/210 min were observed between days 4 and 7 with a third plateau at 1,887 ng/210 min between days 8 and 10. Peak levels of volume and protein occurred between 30 and 90 min following each daily stimulation. In the early days SLI output reached its peak 3.5 h after stimulation, whereas at the end of treatment, synchronous secretion of protein and SLI was observed. These data indicate that important modifications occur in the rate of secretion of protein and SLI in the pancreatic juice in the course of chronic administration of cerulein plus secretin.
开展这些研究是为了评估每隔8小时连续10天给予蛙皮素加促胰液素对大鼠胰液中液体、蛋白质及生长抑素样免疫反应性（SLI）分泌量的影响。在向大鼠肠道输注预先收集的胰液期间，利用清醒的插管大鼠研究胰腺分泌。治疗3天后，液体分泌量达到5.2 ml/210分钟的平台期。蛋白质分泌在5天后稳步增加至218 mg/210分钟，到第7天降至118 mg/210分钟，10天后又升至281 mg/210分钟。SLI释放量在3天后呈阶梯式增加，首个平台期为493 ng/210分钟；在第4天至第7天观察到的值在983至562 ng/210分钟之间，在第8天至第10天有第三个平台期，为1887 ng/210分钟。每次每日刺激后，液体和蛋白质的峰值水平出现在30至90分钟之间。在早期，SLI分泌量在刺激后3.5小时达到峰值，而在治疗结束时，观察到蛋白质和SLI同步分泌。这些数据表明，在长期给予蛙皮素加促胰液素的过程中，胰液中蛋白质和SLI的分泌速率发生了重要改变。
