Peña-Vázquez Gloria Itzel, Serrano-Sandoval Sayra N, Rodríguez-Rodríguez José, Antunes-Ricardo Marilena, Guajardo-Flores Daniel
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología FEMSA, Av. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico; Tecnologico de Monterrey, Institute for Obesity Research, Monterrey, Av. Eugenio Garza Sada 2501 Sur, 64849 Monterrey, NL, Mexico.
Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Av. Eugenio Garza Sada 2501 Sur, Monterrey, NL 64849, Mexico.
Food Chem. 2025 Mar 1;467:142258. doi: 10.1016/j.foodchem.2024.142258. Epub 2024 Nov 29.
Opuntia ficus-indica (OFI) seeds are a rich source of functional lipids, yet research on Mexican cultivars remains limited. This study evaluated the antioxidant and anti-inflammatory properties of lipids extracted through subcritical fluid and supercritical fluid extraction with carbon dioxide (SCE-CO₂ and SFE-CO₂) from Mexican OFI Villanueva and Rojo Vigor seeds with and without enzymatic pretreatment. SCE OFI Villanueva oil showed higher extraction efficiency of linoleic (45.86 mg/g), and oleic (9.86 mg/g) acids purified more than 5.47 and 1.18 times, respectively. Additionally, SCE oils exhibited the highest antioxidant potential (68 %) and anti-inflammatory activity (45 %) at the evaluated doses. In conclusion, SCE-CO₂ enhanced the extraction efficiency of unsaturated fatty acids, improving their potential biological effects, while enzymatic pretreatment did not positively impact on results, suggesting reduced extraction efficiency and bioactivity. These findings suggest that OFI seeds can serve as a valuable source of functional ingredients for the development of value-added food products.
仙人掌（Opuntia ficus-indica，OFI）种子是功能性脂质的丰富来源，但对墨西哥品种的研究仍然有限。本研究评估了通过亚临界流体和超临界二氧化碳流体萃取（SCE-CO₂和SFE-CO₂）从墨西哥OFI品种Villanueva和Rojo Vigor种子中提取的脂质的抗氧化和抗炎特性，其中部分种子经过酶预处理，部分未经过预处理。SCE法提取的Villanueva仙人掌油中亚油酸（45.86 mg/g）和油酸（9.86 mg/g）的提取效率更高，分别比其他方法纯化了5.47倍和1.18倍以上。此外，在所评估的剂量下，SCE法提取的油具有最高的抗氧化潜力（68%）和抗炎活性（45%）。总之，SCE-CO₂提高了不饱和脂肪酸的提取效率，增强了其潜在的生物学效应，而酶预处理对结果没有积极影响，表明提取效率和生物活性降低。这些发现表明，OFI种子可作为开发增值食品的功能性成分的宝贵来源。
