Cheng Tao, Chen Jiandong, Tan Beiping, Chi Shuyan
Aquatic Animal Nutrition and Feed Laboratory, Guangdong Ocean University, Zhanjiang, 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, Guangdong, 524088, China.
Aquatic Animal Nutrition and Feed Laboratory, Guangdong Ocean University, Zhanjiang, 524088, China; Aquatic Animals Precision Nutrition and High-Efficiency Feed Engineering Research Centre of Guangdong Province, Zhanjiang, Guangdong, 524088, China.
Fish Shellfish Immunol. 2025 Feb;157:110072. doi: 10.1016/j.fsi.2024.110072. Epub 2024 Dec 3.
An 8-week feeding trial was conducted to investigate the effects of LA supplementation in a high-fat diet on the growth performance, hepatic antioxidant capacity, and glycogen metabolism of largemouth bass (Micropterus salmoides). Five diets were formulated including control diet (11.55 % crude fat, CF), a high-fat diet (17.80 % crude fat, HF) and three HF diets supplemented with 0.15 %, 0.20 % and 0.25 % LA (HL0.15, HL0.20 and HL0.25, respectively). In this experiments, HL0.15 and HL0.20 could improve the uniform for the growth of the largemouth bass, while adding 0.25 % did not significantly improve growth. The highest viscerosomatic index (VSI) and hepatosomatic index (HSI) were measured in fish fed the HF diet. Compared to the fish fed HF diet, fish fed HL diets showed lower serum total triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST), hepatic malondialdehyde (MDA) and glycogen levels, and higher hepatic catalase (CAT) and glutathione peroxidase (GSH-PX) activities. In addition, the mRNA expression for lipolysis genes in fish liver were increased and for gluconeogenesis and fatty acid synthesis were reduced. The transcript levels of apoptosis-related genes were significantly down-regulated in the liver of largemouth bass in HL0.15 and HL0.20 groups compared to the HF group. Moreover, compared with the HF group, the mRNA expression of pro-inflammatory factors was significantly reduced in HL groups, and the histomorphology of the liver were significantly improved. These results suggested that LA supplementation in high-fat diets could improve lipid utilization, glycogen accumulation, antioxidant capacity of fish liver, thus reduce the adverse effects of high fat diets on fish, and then improve the growth performance of largemouth bass.
进行了一项为期8周的饲养试验，以研究在高脂饲料中添加亚麻酸（LA）对大口黑鲈（Micropterus salmoides）生长性能、肝脏抗氧化能力和糖原代谢的影响。配制了五种饲料，包括对照饲料（粗脂肪含量11.55%，CF）、高脂饲料（粗脂肪含量17.80%，HF）以及三种分别添加0.15%、0.20%和0.25% LA的高脂饲料（分别为HL0.15、HL0.20和HL0.25）。在本试验中，HL0.15和HL0.20可提高大口黑鲈生长的均匀度，而添加0.25% LA并未显著改善生长。饲喂HF饲料的鱼内脏体指数（VSI）和肝体指数（HSI）最高。与饲喂HF饲料的鱼相比，饲喂HL饲料的鱼血清总甘油三酯（TG）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、肝脏丙二醛（MDA）和糖原水平较低，肝脏过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-PX）活性较高。此外，鱼肝中脂肪分解基因的mRNA表达增加，糖异生和脂肪酸合成相关基因的表达降低。与HF组相比，HL0.15和HL0.20组大口黑鲈肝脏中凋亡相关基因的转录水平显著下调。此外，与HF组相比，HL组促炎因子的mRNA表达显著降低，肝脏组织形态学显著改善。这些结果表明，在高脂饲料中添加LA可提高鱼体脂质利用、糖原积累和鱼肝抗氧化能力，从而减轻高脂饲料对鱼的不良影响，进而提高大口黑鲈的生长性能。
