Qiu Yuting, Chen Shiyu, Li Juezhuo, Liu Dong-Ang, Hu Ruiyao, Xu Yue, Chen Keyi, Yuan Jinghua, Zhang Xinling, Li Xiaoping
Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province, Key Laboratory of Artificial Organs and Computational Medicine in Zhejiang Province, Shulan International Medical College, Zhejiang Shuren University, Hangzhou, 310015, PR China.
Wycombe Abbey School Hangzhou, Hangzhou, 311261, PR China.
Clin Chim Acta. 2025 Feb 1;567:120071. doi: 10.1016/j.cca.2024.120071. Epub 2024 Dec 3.
With the frequent outbreaks of viral diseases globally, accurate and rapid diagnosis of viral infections is of significant importance for disease prevention and control. The CRISPR-Cas combined biosensing strategy, as an emergent nucleic acid detection technology, exhibits notable advantages including high specificity, elevated sensitivity, operational simplicity, and cost-effectiveness, thereby demonstrating significant potential in the domain of rapid viral diagnostics. This paper summarizes the principles of the CRISPR-Cas system, the novel biotechnologies, and the latest research progress in virus detection using the combined biosensing strategy. Additionally, this paper discusses the challenges faced by CRISPR-Cas biosensing strategies and outlines future development directions, which provides a reference for further research and clinical applications in the rapid diagnosis of viral infections.
随着全球病毒性疾病的频繁爆发，准确、快速地诊断病毒感染对于疾病的预防和控制至关重要。CRISPR-Cas联合生物传感策略作为一种新兴的核酸检测技术，具有高特异性、高灵敏度、操作简便和成本效益高等显著优势，在快速病毒诊断领域展现出巨大潜力。本文综述了CRISPR-Cas系统的原理、新型生物技术以及利用联合生物传感策略进行病毒检测的最新研究进展。此外，本文还讨论了CRISPR-Cas生物传感策略面临的挑战，并概述了未来的发展方向，为病毒感染快速诊断的进一步研究和临床应用提供参考。
