Li Peimao, Gong Qirui, Wang Dianpeng, Zhang Zhimin, Zhang Wen
Medical Laboratory, Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, 518020, China.
Medical Laboratory College, Hebei North University in China, Zhangjiakou, 075000, Hebei, China.
Sci Rep. 2024 Dec 5;14(1):30402. doi: 10.1038/s41598-024-82140-0.
Benzene is a crucial industrial hydrocarbon, posing significant health risks due to its toxic metabolites like hydroquinone (HQ). This study investigates the role of clusterin (CLU) in benzene toxicity by analyzing its protein and mRNA levels, as well as the expression of Bcl-2 and Bax, to evaluate the feasibility of CLU as a biomarker for chronic benzene poisoning. HL-60 cells were induced to differentiate into neutrophil-like cells using 1% Dimethyl Sulfoxide (DMSO). Enzyme-linked immunosorbent assay (ELISA) and RT-PCR were used to analyze CLU protein and mRNA levels. ELISA was employed to detect sCLU protein content in cell culture supernatants, and western blot was used to assess Bcl-2 and Bax expression. The optimal time for 1% DMSO to induce HL-60 cells into neutrophil-like cells was 48 h. As HQ concentration increased, HL-60 cell viability decreased, CLU protein and sCLU protein levels in the supernatant decreased, CLU mRNA levels decreased, Bcl-2 protein expression decreased, and Bax expression increased. HQ exposure reduces CLU protein concentration and mRNA levels in neutrophil-like cells induced from HL-60 cells, indicating that CLU could be a potential biomarker for chronic benzene poisoning.
苯是一种重要的工业碳氢化合物，由于其有毒代谢产物如对苯二酚（HQ）而对健康构成重大风险。本研究通过分析CLU的蛋白质和mRNA水平以及Bcl-2和Bax的表达，探讨簇集素（CLU）在苯毒性中的作用，以评估CLU作为慢性苯中毒生物标志物的可行性。使用1%二甲基亚砜（DMSO）诱导HL-60细胞分化为中性粒细胞样细胞。采用酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应（RT-PCR）分析CLU蛋白和mRNA水平。用ELISA检测细胞培养上清液中sCLU蛋白含量，用蛋白质免疫印迹法评估Bcl-2和Bax的表达。1%DMSO诱导HL-60细胞分化为中性粒细胞样细胞的最佳时间为48小时。随着HQ浓度的增加，HL-60细胞活力下降，上清液中CLU蛋白和sCLU蛋白水平下降，CLU mRNA水平下降，Bcl-2蛋白表达下降，Bax表达增加。HQ暴露降低了HL-60细胞诱导的中性粒细胞样细胞中CLU蛋白浓度和mRNA水平，表明CLU可能是慢性苯中毒的潜在生物标志物。
