Kozma Kim Zerrin, Park Young Sang, Yang Tae-Jin, Kim Hyun, Lee Yong-Hwan
Department of Agricultural Biotechnology, Seoul National University, Seoul, Korea.
Department of Agriculture, Forestry and Bioresources, Seoul National University, Seoul, Korea.
Sci Rep. 2024 Dec 5;14(1):30381. doi: 10.1038/s41598-024-81942-6.
The intricate interplay between endophytic microorganisms and plants in the upkeep of biodiversity, the stability of communities, and the operation of ecosystems needs to be more adequately extensive. Although root-associated microbial communities of plants have been revealed for the last decade, the understanding of bacterial and fungal communities associated with the roots of medicinal plants remains elusive. To highlight the importance of Panax ginseng Meyer (PG) in our research, we investigated the root endophytic bacterial and fungal communities of Panax ginseng Meyer (PG), alongside Aralia cordata (AC), Angelica gigas (AG), and Peucedanum japonicum (PJ), utilizing amplicon-based community profiling and advanced bioinformatic methodologies. The study aimed to investigate the root-endophytic microbiota of ginseng and three other medicinal plants and identify similarities in microbiome composition across different plant species and families. Results revealed that root-endophytic bacterial and fungal communities were influenced by plant species and phylogenetic differences at the family level. Differential abundance tests and random forest models showed microbial features within the same plant family. PG had a distinctive microbial profile with significant B1653_o_Enterobacterales and F8_o_Helotiales. PG had a core microbiome, B10_Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium, and a more evenly distributed microbial network compared to AG, PJ, and AC. Our research reveals the intricate endophytic microbial communities within the roots of medicinal plants, pinpointing specific taxa that may be pivotal to their medicinal qualities and overall plant health. These insights carry notable implications for future studies, particularly those focused on the endophytes of PG and their secondary metabolites, as they deepen our understanding of plant-microbe interactions and their role in enhancing the plants' therapeutic potential.
内生微生物与植物之间在生物多样性维持、群落稳定性和生态系统运作方面的复杂相互作用，需要得到更充分广泛的研究。尽管在过去十年中已经揭示了植物根系相关的微生物群落，但对于与药用植物根系相关的细菌和真菌群落的理解仍然难以捉摸。为了突出人参（PG）在我们研究中的重要性，我们利用基于扩增子的群落分析和先进的生物信息学方法，研究了人参（PG）以及食用土当归（AC）、当归（AG）和珊瑚菜（PJ）的根系内生细菌和真菌群落。该研究旨在调查人参和其他三种药用植物的根系内生微生物群，并确定不同植物物种和科之间微生物组组成的相似性。结果表明，根系内生细菌和真菌群落受到植物物种和科水平系统发育差异的影响。差异丰度测试和随机森林模型显示了同一植物科内的微生物特征。PG具有独特的微生物特征，有显著的B1653_o_肠杆菌目和F8_o_柔膜菌目。与AG、PJ和AC相比，PG有一个核心微生物组B10_Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium，以及一个分布更均匀的微生物网络。我们的研究揭示了药用植物根系内复杂的内生微生物群落，确定了可能对其药用品质和整体植物健康至关重要的特定分类群。这些见解对未来的研究具有显著意义，特别是那些关注PG内生菌及其次生代谢产物的研究，因为它们加深了我们对植物-微生物相互作用及其在增强植物治疗潜力中作用的理解。
