Key Research Laboratory of Traditional Chinese Medicine Resources Protection, Administration of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
Tongren Municipal People's Hospital, Tongren, China.
Plant Signal Behav. 2021 Nov 2;16(11):1952372. doi: 10.1080/15592324.2021.1952372. Epub 2021 Jul 25.
American ginseng, a valuable medicinal and food plant, is threatened by rot root, which affects its yield and quality. However, limited studies have investigated the changes in soil microbial community and physiochemical properties between healthy and rot root American ginseng. Here, high-throughput sequencing and soil physiochemical properties were used to characterize these changes. The soil physiochemical properties showed significance differences between the soil of healthy and rot root, in which the pH, available potassium, available phosphorus, soil organic carbon and soil organic matter were significantly higher in healthy root soil. Besides, fungal α-diversity was also higher in healthy root soil than that in rot root. Importantly, the dominant fungal genera differed between soils of healthy and rot root of American ginseng, and LEfSe further indicated that six fungal genera ( and ) were significantly enriched in the soil of healthy plants, whereas six fungal genera ( and ) were significantly enriched in the soil of rot root, suggesting that an increase in the relative abundance of these pathogenic fungi (, and ) may be associated with ginseng rot root. Notably, this study is the first to report that an increase in the relative abundances of and in the rot root soil of American ginseng may be associated with the onset of rot root symptoms in this plant. The functional profile prediction showed that the there was a significantly Pathotrophs increase in the rot root soil compared with healthy root soil and Saprotrophs were more abundant in the healthy root soil. Finally, correlation analyses revealed that soil cation exchange capacity was an important factors affecting the composition of rot root of American ginseng soil microbial communities. This study not only used a new approach to explore the new fungal associated with rot root in American ginseng but also excavated the major soil physiochemical properties affecting the microbiome diversity, providing foundation for developing biocontrol strategies against rot root.
西洋参是一种有价值的药用和食用植物，但其受到烂根病的威胁，这会影响其产量和质量。然而，目前关于健康和烂根西洋参之间土壤微生物群落和理化性质变化的研究还很有限。本研究采用高通量测序和土壤理化性质分析方法，对这些变化进行了研究。结果表明，健康和烂根西洋参土壤的理化性质存在显著差异，其中健康根土壤的 pH 值、有效钾、有效磷、土壤有机碳和土壤有机质含量显著较高。此外，健康根土壤中的真菌 α 多样性也高于烂根土壤。重要的是，健康和烂根西洋参土壤中的优势真菌属不同，LEfSe 进一步表明，在健康植物土壤中，有 6 个真菌属（和）显著富集，而在烂根土壤中，有 6 个真菌属（和）显著富集，这表明这些病原真菌（、和）的相对丰度增加可能与西洋参烂根有关。值得注意的是，本研究首次报道，西洋参烂根土壤中 和 的相对丰度增加可能与该植物烂根症状的发生有关。功能预测结果表明，与健康根土壤相比，烂根土壤中的病原菌明显增加，而健康根土壤中的腐生菌则更为丰富。最后，相关性分析表明，土壤阳离子交换量是影响西洋参烂根土壤微生物群落组成的重要因素。本研究不仅采用新方法探索了与西洋参烂根相关的新真菌，还挖掘了影响微生物组多样性的主要土壤理化性质，为开发防治烂根的生物防治策略提供了基础。
