Schryer Aimée, Sica Pietro, Müller-Stöver Dorette, Magid Jakob
University of Copenhagen, Department of Plant and Environmental Sciences, Thorvaldsensvej, 40, 1821, Frederiksberg, Denmark.
MethodsX. 2024 Nov 14;13:103040. doi: 10.1016/j.mex.2024.103040. eCollection 2024 Dec.
Iron oxide-impregnated filter papers (FeO) facilitate the study of soil phosphorus (P) desorption, availability and dynamics. However, many studies use homogenized and saturated shaking FeO soil systems, hindering opportunities to resolve environmentally relevant experimental objectives. This study created and evaluated a static microcosm to study soil P dynamics with FeO papers. The microcosm design is simple: silicone rubber is glued to the bottom of a piece of polyvinyl chloride (PVC) pipe. The soil is packed to a known bulk density into the PVC pipe. Finally, the top of the PVC pipe is sealed with a fine nylon mesh, allowing water exchange to the overlaying filter paper. To validate the systems, we created three proof-of-concept tests. The first determined whether FeO filter papers remove statistically significant P compared to a control. The second test examined the effect of soil water content before finally testing the influence of amendment sources on the amount of P desorbed. Results show that FeO papers paired with the microcosms desorb soil P, while the soil water content and amendment type impact total soil P released. Consequently, the soil microcosm design allows users to test and examine soil P processes with FeO papers under new experimental conditions.•Phosphorus desorption studies using iron oxide-impregnated filter papers are limited by homogenized and saturated extractions. Consequently, a static microcosm design was created to allow for manipulation of experimental factors.•Results from three proof-of-concept tests confirm that using iron oxide-impregnated filter papers with static microcosms promotes phosphorus desorption, allowing for adjustable experimental designs.
氧化铁浸渍滤纸（FeO）有助于研究土壤磷（P）的解吸、有效性和动态变化。然而，许多研究使用均质化和饱和振荡的FeO土壤系统，这阻碍了实现与环境相关的实验目标的机会。本研究创建并评估了一种静态微观系统，用于使用FeO滤纸研究土壤磷动态变化。该微观系统设计简单：将硅橡胶粘在一段聚氯乙烯（PVC）管的底部。将土壤以已知的容重装入PVC管中。最后，用细尼龙网密封PVC管顶部，使水能够与上层滤纸进行交换。为了验证该系统，我们进行了三项概念验证测试。第一项测试确定与对照相比，FeO滤纸是否能去除具有统计学意义的磷。第二项测试检查了土壤含水量的影响，最后测试了改良剂来源对解吸磷量的影响。结果表明，与微观系统结合使用的FeO滤纸能解吸土壤磷，而土壤含水量和改良剂类型会影响释放的总土壤磷量。因此，土壤微观系统设计使使用者能够在新的实验条件下，用FeO滤纸测试和研究土壤磷过程。
• 使用氧化铁浸渍滤纸进行的磷解吸研究受到均质化和饱和提取的限制。因此，创建了一种静态微观系统设计，以允许对实验因素进行操控。
• 三项概念验证测试的结果证实，在静态微观系统中使用氧化铁浸渍滤纸可促进磷解吸，从而实现可调节的实验设计。
