Lehto M, Järvinen M, Nelimarkka O
Arch Orthop Trauma Surg (1978). 1986;104(6):366-70. doi: 10.1007/BF00454432.
The formation of a connective tissue scar was studied autoradiographically 2-42 days following standard contusion injury in the gastrocnemius muscle of 30 rats. The rats were injected intraperitoneally with 3H-labeled proline 4 h before the muscle samples were taken. On day 2 the majority of cells in the injured area were identified as inflammatory without radioactive labeling. Abundant labeling was observed, however, over the extracellular substance, and moderate labeling with 3H-proline in the cells identified as fibroblasts or their precursors. During the first 2 weeks the number of fibroblasts increased and the majority of them were labeled with silver grains, which were also present over the connective tissue matrix. Six weeks after injury only a few fibroblasts showed labeling, and no labeling was observed extracellularly in the connective tissue. Thus, it seems that the synthesis of extracellular connective tissue components in injured skeletal muscle occurs as early as 2 days after trauma, is intensive between days 5 and 21 after trauma, and decreases markedly during the following 3 weeks.
在30只大鼠的腓肠肌进行标准挫伤损伤后2至42天，采用放射自显影法研究了结缔组织瘢痕的形成。在采集肌肉样本前4小时，给大鼠腹腔注射3H标记的脯氨酸。在第2天，损伤区域的大多数细胞被鉴定为炎症细胞，无放射性标记。然而，在细胞外物质上观察到大量标记，并且在被鉴定为成纤维细胞或其前体的细胞中观察到3H-脯氨酸的中度标记。在最初的2周内，成纤维细胞数量增加，其中大多数被银颗粒标记，银颗粒也出现在结缔组织基质上。损伤6周后，只有少数成纤维细胞显示标记，在结缔组织的细胞外未观察到标记。因此，似乎受伤骨骼肌中细胞外结缔组织成分的合成早在创伤后2天就开始，在创伤后第5至21天较为强烈，并在随后的3周内显著减少。
