Almhöjd Ulrica, Fisic Amela, Cevik-Aras Hülya, Tuomi Lisa, Finizia Caterina, Almståhl Annica
Dept of Cariology, Institute of Odontology, Sahlgrenska academy, University of Gothenburg, Sweden.
Section 4- Oral health, Faculty of Odontology, Malmö University, Sweden.
Heliyon. 2024 Oct 9;10(20):e39033. doi: 10.1016/j.heliyon.2024.e39033. eCollection 2024 Oct 30.
to compare saliva proteome of patients before treatment of head and neck cancer and six months post-treatment with controls.
Five dentate patients and five age and gender-matched controls were included. The stimulated salivary secretion rate was determined, and saliva was stored at -80 °C. After thawing, 30 mg of each sample and a reference (aliqouts of all samples) was trypsin digested. The digested peptides were analyzed by mass spectrometry. The relative abundances were transformed to log2 and significant differences determined. Relative abundances of mucins were compared with patient's problems with dry mouth, sticky saliva and swallowing. Data are available via ProteomeXchange with identifier PXD047500.
966 proteins with ≥2 unique peptides were found. Compared with controls, 30 proteins were found in significantly lower relative abundances and 65 in higher at pre-treatment and 38 proteins in significantly lower relative abundances and 34 proteins in higher post-treatment. Regarding proteins from the salivary glands, a significantly lower relative abundance of Cystatins was detected pre-treatment and significantly lower relative abundances of Cystatin, Cysteine-rich secretory protein 3, Lactoperoxidase, Prolactin-inducible protein and Proline-rich protein 4 post-treatment. No clear relation between relative abundance of mucins and dry mouth, sticky saliva and problems with swallowing was detected.
Decreases in several salivary gland proteins post cancer treatment might lead to a reduced defense against oral disorders. Knowledge about changes in saliva proteins in connection with oral cancer treatment is important for planning dental care for these patients.
比较头颈癌患者治疗前及治疗后六个月的唾液蛋白质组与对照组。
纳入五名有牙患者及五名年龄和性别匹配的对照组。测定刺激唾液分泌率，并将唾液储存在-80°C。解冻后，每个样本30mg及一个参考样本（所有样本的等分试样）用胰蛋白酶消化。消化后的肽段通过质谱分析。将相对丰度转换为log2并确定显著差异。将粘蛋白的相对丰度与患者口干、唾液粘稠和吞咽问题进行比较。数据可通过ProteomeXchange获得，标识符为PXD047500。
发现966种蛋白质有≥2个独特肽段。与对照组相比，治疗前有30种蛋白质相对丰度显著降低，65种蛋白质相对丰度升高；治疗后有38种蛋白质相对丰度显著降低，34种蛋白质相对丰度升高。关于唾液腺蛋白质，治疗前检测到胱抑素相对丰度显著降低，治疗后胱抑素、富含半胱氨酸的分泌蛋白3、乳过氧化物酶、催乳素诱导蛋白和富含脯氨酸的蛋白4相对丰度显著降低。未检测到粘蛋白相对丰度与口干、唾液粘稠和吞咽问题之间的明确关系。
癌症治疗后几种唾液腺蛋白质的减少可能导致对口腔疾病的防御能力降低。了解与口腔癌治疗相关的唾液蛋白质变化对于为这些患者规划牙科护理很重要。
