D'Aguì Elisa, Lovisolo Stella, Civera Tiziana, Panebianco Felice
Department of Veterinary Sciences, University of Turin, Grugliasco, Italy.
Ital J Food Saf. 2024 Jul 1;13(4):12296. doi: 10.4081/ijfs.2024.12296. eCollection 2024 Nov 12.
Ascorbic acid is one of the most promising additives for improving fish shelf life, but studies on its antimicrobial activity during product storage are limited. In this experiment, we assessed the effect of ascorbic acid on the preservability of Atlantic mackerel () fillets during storage in ice. Fillets were treated to reach an ascorbic acid concentration of 0.30 mg/g (T1) and 0.15 mg/g (T2). Samples were stored in ice at 0°C and analyzed at 0, 2, 7, 9, and 14 days for total viable count, specific spoilage organisms (SSOs), spp., potentially his-tamine-producing bacteria (HPB), and . Growth curves were constructed using the DMfit tool of Combase, and the shelf life was estimated in relation to microbiological limits reported in the literature. Sensory evaluation was performed using a quality index method scheme. At 7 and 9 days of storage, treated samples exhibited bacterial counts from 0.5 to 1.7 Log CFU/g lower than controls, with a logarithmic reduction proportional to the additive concentration. The antimicrobial action appeared to diminish after 14 days. A consistent effect was observed for potentially HPB, with counts of 1.7 Log CFU/g lower in T1 samples compared to controls at 9 days. The moderate effect on SSOs limited the estimated shelf life, as the critical limit was reached after 7 days and 8.4 days (T1 and T2). Ascorbic acid positively impacted the microbiological characteristics of mackerel fillets. Further investigation into the decay dynamics of the additive in fish products during storage is advisable.
抗坏血酸是改善鱼类货架期最有前景的添加剂之一,但关于其在产品储存期间抗菌活性的研究有限。在本实验中,我们评估了抗坏血酸对大西洋鲭鱼鱼片在冰藏期间保鲜性能的影响。鱼片经处理后抗坏血酸浓度达到0.30毫克/克(T1)和0.15毫克/克(T2)。样品在0°C冰藏,并在0、2、7、9和14天分析总活菌数、特定腐败菌(SSO)、 菌属、潜在产组胺细菌(HPB)以及 。使用Combase的DMfit工具构建生长曲线,并根据文献报道的微生物限度估计货架期。采用质量指数法方案进行感官评价。在储存7天和9天时,处理后的样品细菌计数比对照低0.5至1.7 Log CFU/克,对数减少量与添加剂浓度成正比。14天后抗菌作用似乎减弱。对于潜在的HPB观察到一致的效果,在9天时T1样品中的计数比对照低1.7 Log CFU/克。对SSO的适度影响限制了估计的货架期,因为在7天和8.4天(T1和T2)后达到了临界限度。抗坏血酸对鲭鱼鱼片的微生物特性有积极影响。建议进一步研究该添加剂在鱼产品储存期间的衰减动态。