Mina Sohana Akter, Debnath Pabitra, Hossain A K M Zakir, Hasan Md Zahid, Azad Chowdhury A M Masudul
Laboratory of Microbial and Cancer Genomics, Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences, University of Chittagong, Chittagong, 4331, Bangladesh.
Heliyon. 2024 Nov 19;10(22):e40523. doi: 10.1016/j.heliyon.2024.e40523. eCollection 2024 Nov 30.
Contaminated water poses a significant public health risk due to waterborne microbial diseases, with typhoid caused by Typhi being the most prevalent bacterial waterborne disease in Bangladesh. This study aimed to assess the presence of antibiotic-resistant genes and their patterns in Typhi isolated from drinking water sources in Chattogram City, Bangladesh. Out of 150 samples analyzed, 10 isolates were suspected to be Typhi by selective plating and biochemical test and 8 were confirmed using PCR amplification of the gene and subsequent sequencing. Phylogenetic analysis provided insights into the genetic diversity and relationships among the identified isolates. The study revealed the presence of Typhi in certain drinking water sources, indicating a potential threat to public health. The presence of antibiotic-resistant genes, including , , , and , in the genomic and plasmid DNA of Typhi isolates emphasizes the role of contaminated water in spreading antibiotic resistance. All 8 isolates were found to contain at least one antibiotic-resistant gene highlighting the widespread presence of resistance elements. This raises serious concerns about the potential impact on the effectiveness of typhoid treatment. Antibiotic susceptibility testing using the disk diffusion assay confirmed multidrug resistance (MDR) in 87.5 % of the Typhi isolates, highlighting the urgent need to address the transmission of antibiotic-resistant genes through drinking water sources. The results confirm the widespread environmental transmission of antibiotic resistance in the region and emphasize the potential consequences for the efficacy of typhoid treatment.
受污染的水因水传播的微生物疾病而对公众健康构成重大风险，由伤寒杆菌引起的伤寒是孟加拉国最普遍的细菌性水传播疾病。本研究旨在评估从孟加拉国吉大港市饮用水源分离出的伤寒杆菌中抗生素抗性基因的存在情况及其模式。在分析的150个样本中，通过选择性平板培养和生化试验，有10株分离株疑似为伤寒杆菌，通过该基因的PCR扩增及后续测序，8株得到确认。系统发育分析为已鉴定分离株之间的遗传多样性和关系提供了见解。该研究揭示了某些饮用水源中存在伤寒杆菌，表明对公众健康存在潜在威胁。伤寒杆菌分离株的基因组和质粒DNA中存在抗生素抗性基因，包括、、和，这突出了受污染水在传播抗生素抗性中的作用。所有8株分离株均被发现至少含有一个抗生素抗性基因，这凸显了抗性元件的广泛存在。这引发了对伤寒治疗效果潜在影响的严重担忧。使用纸片扩散法进行的抗生素敏感性测试证实，在87.5%的伤寒杆菌分离株中存在多重耐药性（MDR），这突出了迫切需要解决通过饮用水源传播抗生素抗性基因的问题。结果证实了该地区抗生素抗性的广泛环境传播，并强调了对伤寒治疗效果的潜在影响
