He Zhien, Huang Yi, Li Wei, Zhang Huanhuan, Cao Ruobing, Ali Md Roushan, Dai Yuanyuan, Lu Huaiwei, Wang Wanying, Niu Qiuhong, Sun Baolin, Li Yujie
Department of Oncology, The First Affiliated Hospital of University of Science and Technology of China, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, China.
Department of Cancer Epigenetics Program, The First Affiliated Hospital of University of Science and Technology of China, Hefei, Anhui, China.
Antimicrob Agents Chemother. 2025 Jan 31;69(1):e0126224. doi: 10.1128/aac.01262-24. Epub 2024 Dec 6.
The multidrug-resistant clonal complex 92 is spreading worldwide due to its high-frequency gene mutation and recombination, posing a significant threat to global medical and health safety. Between November 2021 and April 2022, a total of 132 clinical isolates were collected from a tertiary hospital in China. Their growth ability and virulence of these isolates were assessed using growth curve analyses and the infection model. The genetic characteristics of the isolates were further examined through whole-genome sequencing. ST1791/ST2 isolates represented the largest proportion of isolates in our collection and exhibited the highest growth rate and strongest virulence among all sequence types (STs) analyzed. Whole-genome sequences from 14,159 clinical isolates were collected from the National Center for Biotechnology Information database, and only nine ST1791/ST2 isolates were detected. Comparative genomic analysis revealed that ST1791/ST2 carried 11 unique genes, 5 of which were located within the capsular polysaccharide synthesis () gene cluster. Single nucleotide polymorphisms (SNPs) between ST1791/ST2 and other isolates were primarily found in the cps gene cluster. Among the other isolates, ST195/ST2 and ST208/ST2 exhibited the smallest SNP differences from ST1791/ST2, while ST195/ST2 and ST1486/ST2 had high homology. The ST1791/ST2 strain in Anhui, China, displayed significant homology with ST195/ST2, ST208/ST2, and ST1486/ST2 isolates. Compared to other isolates in this study, ST1791/ST2 exhibited strong growth ability and virulence. Therefore, preventing the further spread of ST1791/ST2 should be a top public health priority.
多重耐药克隆复合体92因其高频基因突变和重组而在全球范围内传播，对全球医疗卫生安全构成重大威胁。2021年11月至2022年4月，从中国一家三级医院共收集了132株临床分离株。使用生长曲线分析和感染模型评估了这些分离株的生长能力和毒力。通过全基因组测序进一步检测了分离株的遗传特征。ST1791/ST2分离株在我们收集的分离株中占比最大，在所有分析的序列类型（STs）中表现出最高的生长速率和最强的毒力。从国家生物技术信息中心数据库收集了14159株临床分离株的全基因组序列，仅检测到9株ST1791/ST2分离株。比较基因组分析显示，ST1791/ST2携带11个独特基因，其中5个位于荚膜多糖合成（）基因簇内。ST1791/ST2与其他分离株之间的单核苷酸多态性（SNP）主要存在于cps基因簇中。在其他分离株中，ST195/ST2和ST208/ST2与ST1791/ST2的SNP差异最小，而ST195/ST2和ST1486/ST2具有高度同源性。中国安徽的ST1791/ST2菌株与ST195/ST2、ST208/ST2和ST1486/ST2分离株表现出显著同源性。与本研究中的其他分离株相比，ST1791/ST2表现出较强的生长能力和毒力。因此，防止ST1791/ST进一步传播应是公共卫生的首要任务。
