Department of Oncology, The First Affiliated Hospital, University of Science and Technology of China, Hefei, China.
Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, 510080, China.
BMC Genomics. 2022 Jan 31;23(1):88. doi: 10.1186/s12864-022-08301-5.
Mobile colistin resistance like gene (mcr-like gene) is a new type of polymyxin resistance gene that can be horizontally transferred in the Enterobacteriaceae. This has brought great challenges to the treatment of multidrug-resistant Escherichia coli and K. pneumoniae.
K. pneumoniae 16BU137 and E. coli 17MR471 were isolated from the bus and subway handrails in Guangzhou, China. K. pneumoniae 19PDR22 and KP20191015 were isolated from patients with urinary tract infection and severe pneumonia in Anhui, China. Sequence analysis indicated that the mcr-1.1 gene was present on the chromosome of E. coli 17MR471, and the gene was in the gene cassette containing pap2 and two copies of ISApl1.The mcr-1.1 was found in the putative IncX4 type plasmid p16BU137_mcr-1.1 of K. pneumoniae 16BU137, but ISApl1 was not found in its flanking sequence. Mcr-8 variants were found in the putative IncFIB/ IncFII plasmid pKP20191015_mcr-8 of K. pneumoniae KP20191015 and flanked by ISEcl1 and ISKpn26.
This study provides timely information on Enterobacteriaceae bacteria carrying mcr-like genes, and provides a reference for studying the spread of mcr-1 in China and globally.
移动多粘菌素耐药基因（mcr-like 基因）是一种新型的多粘菌素耐药基因，可在肠杆菌科中水平转移。这给多药耐药性大肠杆菌和肺炎克雷伯菌的治疗带来了巨大挑战。
从中国广州的公共汽车和地铁扶手上分离到肺炎克雷伯菌 16BU137 和大肠杆菌 17MR471，从中国安徽的尿路感染和重症肺炎患者中分离到肺炎克雷伯菌 19PDR22 和 KP20191015。序列分析表明，mcr-1.1 基因存在于大肠杆菌 17MR471 的染色体上，并且该基因位于包含 pap2 和两个 ISApl1 拷贝的基因盒中。mcr-1.1 存在于肺炎克雷伯菌 16BU137 的假定 IncX4 型质粒 p16BU137_mcr-1.1 中，但在其侧翼序列中未发现 ISApl1。在肺炎克雷伯菌 KP20191015 的假定 IncFIB/IncFII 质粒 pKP20191015_mcr-8 中发现了 Mcr-8 变体，其两侧为 ISEcl1 和 ISKpn26。
本研究及时提供了携带 mcr-like 基因的肠杆菌科细菌的信息，为研究 mcr-1 在我国及全球的传播提供了参考。
