Brigham K L, Harris T R, Bowers R E, Roselli R J
Lymphology. 1979 Sep;12(3):177-90.
In chronically instrumented unanesthetized sheep, we measured steady-state hemodynamic and lung lymph responses to mechanically increased pressure and to intravenous infusions of histamine, Pseudomonas bacteria and E. coli endotoxin. Histamine, Pseudomonas bacteria and E. coli endotoxin caused exchanging vessel permeability to increase, as evidenced by high flows of protein rich lung lymph. This contrasts to the effects of increased pressure where lymph protein concentration falls as lymph flow increases. Microvascular sieving of proteins less than 100 Angstrom radius persisted in all increased permeability states, but with endotoxin, lymph clearance of larger proteins increased much more than with histamine or Pseudomonas. We compared several approaches to quantitative interpretations of lymph data and found that direct methods for calculating permeability-surface area products and reflection coefficients for proteins produced values which were difficult to interpret, probably because fundamental assumptions of the methods were violated in our experiments. A mathematical model based on multiple pore theory produced more plausible coefficients.
在长期植入仪器的未麻醉绵羊中，我们测量了对机械性增压以及静脉注射组胺、铜绿假单胞菌和大肠杆菌内毒素的稳态血流动力学和肺淋巴反应。组胺、铜绿假单胞菌和大肠杆菌内毒素导致交换血管通透性增加，富含蛋白质的肺淋巴高流量证明了这一点。这与压力增加的影响形成对比，压力增加时淋巴蛋白浓度随淋巴流量增加而下降。在所有通透性增加的状态下，半径小于100埃的蛋白质的微血管筛滤持续存在，但在内毒素作用下，较大蛋白质的淋巴清除率增加幅度远大于组胺或铜绿假单胞菌作用时。我们比较了几种对淋巴数据进行定量解释的方法，发现计算蛋白质通透表面积乘积和反射系数的直接方法得出的值难以解释，可能是因为这些方法的基本假设在我们的实验中被违反了。基于多孔理论的数学模型产生了更合理的系数。
