Vaughan T R, Erdmann A J, Brigham K L, Woolverton W C, Weidner W J, Staub N C
Lymphology. 1979 Sep;12(3):217-23.
In 16 unanesthetized sheep with chronic lung lymph fistulas we measured pulmonary vascular pressures, lymph flow, lymph and plasma total protein and albumin concentration. We determined the rate of equilibration of radioiodinated albumin between plasma and lung interstitial fluid (lung lymph) in three steady-state conditions; baseline (n = 14), increased pulmonary microvascular pressure (n = 9) and increased microvascular permeability (n = 4). The tracer protein equilibration proceeded according to single compartment wash-in kinetics in all experiments. Lung lymph flow averaged 5.3 +/- 2.8 (S.D.)ml/h under baseline conditions, 16.1 +/- 10.6 ml/h during increased pressure and 37.3 +/- 29.4 ml/h during increased permeability. The half time of equilibration averaged 2.9 +/- 1.0 h, 2.2 +/- 1.0 h and 0.7 +/- 0.2 h, respectively. Lung interstitial fluid equilibrates with plasma proteins more rapidly than most other organs. The marked difference between increased permeability and the other conditions demonstrates the sensitivity of this method. No evidence was obtained that any tracer protein entered lung lymph within the caudal mediastinal lymph node.
在16只患有慢性肺淋巴瘘的未麻醉绵羊中，我们测量了肺血管压力、淋巴流量、淋巴及血浆总蛋白和白蛋白浓度。我们在三种稳态条件下测定了放射性碘化白蛋白在血浆与肺间质液（肺淋巴）之间的平衡速率；基线状态（n = 14）、肺微血管压力升高（n = 9）和微血管通透性增加（n = 4）。在所有实验中，示踪蛋白的平衡均按照单室灌洗动力学进行。在基线条件下，肺淋巴流量平均为5.3±2.8（标准差）ml/h，压力升高时为16.1±10.6 ml/h，通透性增加时为37.3±29.4 ml/h。平衡的半衰期分别平均为2.9±1.0 h、2.2±1.0 h和0.7±0.2 h。肺间质液与血浆蛋白的平衡比大多数其他器官更快。通透性增加与其他条件之间的显著差异证明了该方法的敏感性。未获得任何证据表明任何示踪蛋白进入了后纵隔淋巴结内的肺淋巴。
