Stecher V J, Kaplan J E, Connolly K, Mielens Z, Saelens J K
Arthritis Rheum. 1986 Mar;29(3):394-9. doi: 10.1002/art.1780290313.
Recent evidence suggests that fibronectin (Fn), a high molecular weight glycoprotein, may be used as an indicator protein in rats with adjuvant-induced arthritis. Rocket immunoelectrophoresis, using purified goat anti-rat Fn, provided a specific and sensitive means of measuring plasma Fn in rats during the development of various inflammatory disease states. It was shown that normal rat plasma Fn levels of approximately 400 micrograms/ml double within 24 hours after injection of adjuvant. Plasma Fn levels in this model of chronic systemic inflammatory joint disease were tracked for more than 4 months and remained significantly higher than normal. On the other hand, a carrageenan-induced inflammatory response in the pleural cavity of rats resulted in a large local accumulation of leukocytes, but no change in plasma Fn levels. A carrageenan-induced model of acute inflammation resulted in increased paw swelling within 6 hours and enhanced plasma Fn levels within 24 hours; plasma Fn levels returned to normal within 1 week. Quantitation of plasma Fn levels in the rat may provide a useful biochemical parameter for the study of chronic systemic inflammatory diseases.
最近有证据表明，纤连蛋白（Fn），一种高分子量糖蛋白，可用作佐剂诱导性关节炎大鼠的指示蛋白。使用纯化的山羊抗大鼠Fn进行火箭免疫电泳，为在各种炎症性疾病状态发展过程中测量大鼠血浆Fn提供了一种特异且灵敏的方法。结果表明，正常大鼠血浆Fn水平约为400微克/毫升，在注射佐剂后24小时内翻倍。在这个慢性全身性炎症性关节病模型中，血浆Fn水平被追踪了4个多月，且仍显著高于正常水平。另一方面，角叉菜胶诱导的大鼠胸腔炎症反应导致白细胞大量局部聚集，但血浆Fn水平没有变化。角叉菜胶诱导的急性炎症模型在6小时内导致爪肿胀增加，在24小时内血浆Fn水平升高；血浆Fn水平在1周内恢复正常。定量测定大鼠血浆Fn水平可能为慢性全身性炎症疾病的研究提供一个有用的生化参数。
