Lay Jiunn-Jyi, Huang Yu-Tzu, Han Chang-Lung, Zhong Wei-Zhen
Department of Safety, Health and Environmental Engineering, National Kaohsiung University of Science and Technology, 1, University Rd., Yanchau, Kaohsiung, 811, Taiwan, ROC.
Department of Chemical Engineering, Chung-Yuan Christian University, 200 Chung Pei Road, Jhong-Li, 32023, Taiwan, ROC.
J Environ Manage. 2025 Jan;373:123604. doi: 10.1016/j.jenvman.2024.123604. Epub 2024 Dec 6.
This study investigates the enhancement of diesel degradation in contaminated soil through the synergistic effects of functional microbiomes and phytoremediation, emphasizing increased enzyme activity. The approach integrates a hydrogen-producing microbiome (HMb) with phytoremediation techniques. Observations revealed changes in soil conditions, including increases in moisture levels from 12.5% to 20% and a shift in pH from 6.9 to an alkaline range of 8.0-8.5 due to the treatment. Organic matter content also improved, supporting microbial activity. These modifications were closely monitored to evaluate their impact on microbial growth and enzyme activity. The findings showed that total petroleum hydrocarbons (TPH) in diesel-contaminated soil decreased by 78.1% using the combined HMb and phytoremediation method. This decrease was markedly higher than the 30.4% achieved through water drenching and the 30.9% with HMb alone. Central to this success were Clostridium sp. and Sporolactobacillus sp., which played essential roles in hydrocarbon degradation. Improved soil conditions supported an increase in microbial populations, with bacterial counts peaking at 6.0 x 10 by day 4, enhancing degradation. Additionally, Bermuda grass survival rates increased to 35% by day 35. In the HMb and planting combination, amylase activity peaked at 100% by day 10, significantly aiding degradation, although it later decreased to 1% by day 35. This research presents a robust strategy for diesel-contaminated soil remediation, highlighting significant advancements in microbial growth and degradation efficiency.
本研究通过功能微生物群落与植物修复的协同作用，研究受污染土壤中柴油降解的增强情况，重点关注酶活性的提高。该方法将产氢微生物群落（HMb）与植物修复技术相结合。观察结果显示，土壤条件发生了变化，包括处理后土壤湿度从12.5%增加到20%，pH值从6.9转变为8.0 - 8.5的碱性范围。有机质含量也有所改善，有利于微生物活动。对这些变化进行了密切监测，以评估它们对微生物生长和酶活性的影响。研究结果表明，采用HMb与植物修复相结合的方法，柴油污染土壤中的总石油烃（TPH）降低了78.1%。这一降幅明显高于通过浇水实现的30.4%以及仅使用HMb时的30.9%。成功的关键在于梭菌属和芽孢乳杆菌属，它们在烃类降解中发挥了重要作用。改善后的土壤条件支持了微生物种群的增加，细菌数量在第4天达到峰值6.0×10，促进了降解。此外，到第35天，百慕大草的存活率提高到了35%。在HMb与种植的组合中，淀粉酶活性在第10天达到峰值100%，显著促进了降解，不过到第35天它降至1%。这项研究为柴油污染土壤修复提出了一种强有力的策略，突出了微生物生长和降解效率方面的重大进展。
