Xu Yaping, Wu Hongchun, Jiang Jingxiu, Ye Lingqun, Hao Kaili, Han Kunjun, Hu Shijun, Lei Wei, Guo Zhikun
The 7th People's Hospital of Zhengzhou, Zhengzhou 450000, Henan, China.
Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Suzhou 215000, Jiangsu, China.
Stem Cell Res. 2025 Feb;82:103619. doi: 10.1016/j.scr.2024.103619. Epub 2024 Dec 1.
Long Intergenic Non-Protein Coding RNA 1405 (LINC01405), with known elevated expression in muscle, has been linked to a number of musculo-skeletal conditions. By utilizing the CRISPR/Cas9 gene editing system, we generated a LINC01405 knockout human embryonic stem cell (hESC) line. This line remains human stem cell-like morphology and pluripotency, exhibits a normal karyotype, and can differentiate into cells from all three germ layers. This cell line will be an invaluable model for the research on LINC01405's role in normal development of cardiac and skeletal muscle, and their diseases.
长链基因间非编码RNA 1405（LINC01405）在肌肉中表达已知升高，与多种肌肉骨骼疾病有关。通过利用CRISPR/Cas9基因编辑系统，我们构建了一个LINC01405基因敲除的人胚胎干细胞（hESC）系。该细胞系保持人干细胞样形态和多能性，具有正常核型，并且能够分化为所有三个胚层的细胞。该细胞系将成为研究LINC01405在心肌和骨骼肌正常发育及其疾病中作用的宝贵模型。
