Wang Zhixiu, Gu Shaoxuan, Lv Wen, Chen Jiayi, Xue Min, Liu Suli, Mao Jiaming, Chen Guohong
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Microorganisms. 2025 Sep 17;13(9):2168. doi: 10.3390/microorganisms13092168.
, a Gram-negative bacterium prevalent in aquatic environments and also frequently isolated from livestock and poultry, was investigated through integrated whole-genome sequencing and functional analyses. We deciphered the pathogenic mechanisms of CA-HZ1, a highly virulent strain isolated from a novel piscine host, revealing a complete genome assembly with a 3.49 Mb circular chromosome and 311 kb plasmid housing 3247 predicted protein-encoding genes. Critical genomic features included 496 virulence factors and 225 antibiotic resistance genes. Pathogenicity analysis indicated that was responsible for disease outbreaks. Antimicrobial susceptibility tests showed resistance to various drugs, such as kanamycin, erythromycin, and penicillin. Histopathological examination showed significant alterations in the infected hosts. Quantitative real-time PCR (qRT-PCR) was carried out to analyze immune-related gene (IL-6, IL-1β, IL-21, STAT1, and HSP70) levels in liver and intestinal tissues, demonstrating the potent immunity triggered by infection. An analysis of the liver transcriptome revealed that has the potential to influence the cellular composition, molecular functions, and biological processes. Collectively, this study describes the genomic basis underlying both the pathogenic potential and hypervirulence of CA-HZ1, establishing a foundational framework for investigating its broad host tropism and immune response.
一种革兰氏阴性菌，在水生环境中普遍存在，也经常从家畜和家禽中分离出来，通过全基因组测序和功能分析进行了研究。我们解析了从一种新型鱼类宿主分离出的高毒力菌株CA-HZ1的致病机制，揭示了一个完整的基因组组装，包含一个3.49 Mb的环状染色体和一个311 kb的质粒，其中含有3247个预测的蛋白质编码基因。关键的基因组特征包括496个毒力因子和225个抗生素抗性基因。致病性分析表明，该菌是疾病爆发的原因。药敏试验显示对卡那霉素、红霉素和青霉素等多种药物耐药。组织病理学检查显示感染宿主有显著变化。进行了定量实时PCR（qRT-PCR）分析肝脏和肠道组织中免疫相关基因（IL-6、IL-1β、IL-21、STAT1和HSP70）的水平，证明了该菌感染引发的强大免疫反应。肝脏转录组分析表明，该菌有可能影响细胞组成、分子功能和生物学过程。总体而言，本研究描述了CA-HZ1致病潜力和高毒力的基因组基础，为研究其广泛的宿主嗜性和免疫反应建立了一个基础框架。
