The combined metabolism and transcriptome of tail muscles reveal the effects of antimony pulse exposure on swimming behavior of Pelophylax nigromaculatus tadpoles.

Due to the periodic emission of pollutants, the exposure mode of contaminants in water bodies is mostly pulse exposure, and the toxic effects of fluctuating exposure on aquatic animals are not consistent with traditional toxicological experiments of constant exposure. The toxic effects of heavy metal antimony (Sb) on the swimming behavior of Pelophylax nigromaculatus tadpoles after pulse exposure (PESb) and continuous exposure (CESb) for 28 days were explored. The mechanisms were analyzed from the perspectives of tail muscle metabolism and transcriptomics. Compared to the control group, PESb and CESb decreased the average speed of P. nigromaculatus tadpoles by 25.72 % and 18.08 %, respectively. PESb and CESb led to changes in 70 and 24 metabolites of tail muscle, respectively. PESb led to alterations in metabolic pathways related to pyrimidine metabolism, arginine biosynthesis, and glycerophospholipid metabolism. In contrast, CESb altered metabolic pathways such as alanine, aspartate, and glutamate metabolism. Compared to the control, 1225 and 1139 DEGs were identified for PESb and CESb, respectively. These DEGs were mainly associated with functions such as immune response, DNA replication, protein digestion, and absorption. It can be seen that PESb and CESb can alter the metabolism and transcriptome of the tail muscle of P. nigromaculatus tadpoles, leading to differential expression of individual movements.