Xia Yuqiong, Liu Chang, Zhao Xuejuan, Wu Keyun, Cao Jianxia, Cao Yutian, Zhu Cheng, Zhang Xianghan
Engineering Research Center of Molecular- and Neuro-imaging of Ministry of Education, School of Life Science and Technology, Xidian University, Xi'an, Shaanxi, 710126, China.
Guangzhou Institute of Technology, Xidian University, Guangzhou, Guangdong, 510555, China.
Mater Today Bio. 2024 Nov 19;29:101345. doi: 10.1016/j.mtbio.2024.101345. eCollection 2024 Dec.
Natural enzyme-based catalytic cascades have garnered increasing attention in cancer therapy, but their clinical utility is greatly limited due to loss of function during delivery. Here, we developed an enzyme delivering nanoplatform (GCI@RPCM) with great stability and achieve NIR-triggered enzyme dynamic therapy. This nanoplatform is created with encapsulation of nature enzymes (glucose oxidase and chloroperoxidase) and photothermal agent (indocyanine green) within tumor targeting and thermo-responsive phase change materials (RPCMs). With NIR irradiation for 10 min, GCI@RPCM can release 41 % of the enzymes and generate abundant reactive oxygen species (ROS), which showed significant tumor cell inhibition. After intravenous injection, GCI@RPCM can efficiently accumulate at the tumor site and local NIR treatment resulted in complete tumor eradication without detectable systemic toxicity. This study provides a highly stable and NIR-controllable smart delivery system and achieve enzyme dynamic therapy for enhanced breast cancer therapy.
基于天然酶的催化级联反应在癌症治疗中受到越来越多的关注，但其临床应用因递送过程中的功能丧失而受到极大限制。在此，我们开发了一种具有高稳定性的酶递送纳米平台（GCI@RPCM），并实现了近红外触发的酶动态疗法。该纳米平台通过将天然酶（葡萄糖氧化酶和氯过氧化物酶）和光热剂（吲哚菁绿）封装在肿瘤靶向和热响应相变材料（RPCM）中构建而成。经10分钟近红外照射后，GCI@RPCM可释放41%的酶并产生大量活性氧（ROS），这显示出对肿瘤细胞的显著抑制作用。静脉注射后，GCI@RPCM可有效积聚在肿瘤部位，局部近红外治疗可实现肿瘤完全根除，且未检测到全身毒性。本研究提供了一种高度稳定且近红外可控的智能递送系统，并实现了用于增强乳腺癌治疗的酶动态疗法。
