Ninchan Boontiwa, Chauywongyart Parimitta, Utapong Teerawat, Thamrongsiripak Nuatawan
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Sugars and Derivatives Analytical Laboratory, Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
Int J Biomater. 2024 Nov 30;2024:8875341. doi: 10.1155/ijbm/8875341. eCollection 2024.
High-energy nonthermal processes (irradiation) are an interesting technique for depolymerization. Gum tragacanth (GT) is a heteropolysaccharide composed of various sugars that are beneficial in the food and pharmaceutical industries. This study investigated the effects of different gamma irradiation doses (2.5, 5, 10, 20, 100, 500, 1,000, and 2000 kGy) on GT properties, considering both structural and physicochemical changes. The results confirmed that gamma irradiation influenced depolymerization with increases in monosaccharides (L-arabinose, D-galactose, D-glucose, D-xylose, L-fucose, L-rhamnose) and the percentage of degradation. Fourier transform infrared (FTIR) spectroscopy analysis indicated that structural changes occurred, with more free O-H and C-O bonding, including the carboxylic group (COOH) in the degraded molecules after irradiation. The changes in physicochemical properties were lower viscosity and a color change under gamma irradiation. The property changes in the GT were clearly related to an increased dose of gamma rays. In summary, there was comprehensive GT degradation following exposure using different increasing doses of gamma radiation, with some concomitant property changes in the GT.
高能非热过程（辐照）是一种有趣的解聚技术。刺梧桐胶（GT）是一种由多种糖类组成的杂多糖，在食品和制药行业中具有益处。本研究考察了不同剂量的γ射线辐照（2.5、5、10、20、100、500、1000和2000 kGy）对GT性质的影响，同时考虑了其结构和物理化学变化。结果证实，γ射线辐照会导致解聚，单糖（L-阿拉伯糖、D-半乳糖、D-葡萄糖、D-木糖、L-岩藻糖、L-鼠李糖）增加以及降解百分比上升。傅里叶变换红外（FTIR）光谱分析表明，辐照后结构发生了变化，降解分子中有更多的游离O-H和C-O键，包括羧基（COOH）。γ射线辐照下物理化学性质的变化表现为较低的粘度和颜色变化。GT的性质变化与γ射线剂量增加明显相关。总之，使用不同递增剂量的γ射线辐照后，GT发生了全面降解，同时GT的一些性质也随之改变。
