Cheng Zixiang, Li Ke, Liu Hongxiu, Wei Xingen, Yin Tao, Xing Xin, Han Lida, Sui Yi
State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
aBIOTECH. 2024 Sep 27;5(4):502-506. doi: 10.1007/s42994-024-00180-6. eCollection 2024 Dec.
Sorghum, the fifth largest global cereal crop, comprises various types, such as grain, sweet, forage, and biomass sorghum, delineated by their designated end uses. Among these, sweet sorghum ( (L.) Moench) stands out for its unique versatility, exceptional abiotic stress tolerance and large biomass serving the multi-purpose of high-sugar forage, syrup, and biofuel production. Despite its significance, functional genomic research and biotechnological breeding in sweet sorghum are still in nascent stages, necessitating more efficient genetic transformation and genome-editing techniques. This study unveils Gaoliangzhe (GZ), an elite sweet sorghum variety for heightened resistance to salinity and drought. Through the establishment of an -mediated genetic transformation and CRISPR/Cas9-based genome-editing system in GZ, a breakthrough is achieved. Using genome-editing technology, we first produced a fragrant sweet sorghum line by targeting the () gene. Our results establish a strong foundation for further functional genomic research and biotechnological breeding of sweet-sorghum varieties.
The online version contains supplementary material available at 10.1007/s42994-024-00180-6.
高粱是全球第五大谷类作物，包括多种类型，如籽粒高粱、甜高粱、饲用高粱和生物质高粱，根据其指定的最终用途划分。其中，甜高粱（(L.) Moench）因其独特的多功能性、出色的非生物胁迫耐受性和大量生物质而脱颖而出，可用于生产高糖饲料、糖浆和生物燃料等多种用途。尽管其具有重要意义，但甜高粱的功能基因组研究和生物技术育种仍处于起步阶段，需要更高效的遗传转化和基因组编辑技术。本研究揭示了高粱哲（GZ），这是一个对盐和干旱具有更高抗性的优良甜高粱品种。通过在GZ中建立介导的遗传转化和基于CRISPR/Cas9的基因组编辑系统，取得了突破。利用基因组编辑技术，我们首先通过靶向()基因培育出了一个香型甜高粱品系。我们的结果为甜高粱品种的进一步功能基因组研究和生物技术育种奠定了坚实基础。
在线版本包含可在10.1007/s42994-024-00180-6获取的补充材料。
