通过染色体显带分析研究人类卵巢癌中克隆变异体的产生。

Generation of clonal variants in a human ovarian carcinoma studied by chromosome banding analysis.

作者信息

Trent J M, Thompson F H, Buick R N

出版信息

Cancer Genet Cytogenet. 1985 Jan 1;14(1-2):153-61. doi: 10.1016/0165-4608(85)90226-2.

Abstract

We have utilized detailed chromosome banding analysis to document the selection of variant genetic clones existing in cell populations obtained from a patient with ovarian adenocarcinoma. Cytogenetic analysis was performed on two samples from the patient's malignant ascites obtained over a 9-month interval. Tumor cell lines were also established in monolayer culture from each ascites sample, analyzed by chromosome banding techniques, and compared to the clonogenic population grown from each ascites specimen. Appearance of karyotypically distinct populations were identified from both the in vivo tumor and the tumor cell lines. Tumor colony-forming units (TCFU) evidenced minimal karyotypic change between the original ascites tumor and the ascites sample obtained after a 9-month period in vivo. In contrast, the cell lines derived from these two ascites samples demonstrated markedly different karyotypic populations between samples and progressive chromosome change within each sample following prolonged in vitro growth.

摘要

我们利用详细的染色体显带分析来记录从一名卵巢腺癌患者获得的细胞群体中存在的变异基因克隆的选择情况。对该患者在9个月期间获得的两份恶性腹水样本进行了细胞遗传学分析。还从每个腹水样本中建立了单层培养的肿瘤细胞系，通过染色体显带技术进行分析，并与从每个腹水标本中生长的克隆形成群体进行比较。在体内肿瘤和肿瘤细胞系中均鉴定出核型不同的群体。肿瘤集落形成单位（TCFU）表明，原始腹水肿瘤与体内9个月后获得的腹水样本之间的核型变化极小。相比之下，来自这两个腹水样本的细胞系在样本之间显示出明显不同的核型群体，并且在体外长期生长后，每个样本内都有渐进性的染色体变化。