Trent J M, Stanisic T, Olson S
J Urol. 1984 Jan;131(1):146-51. doi: 10.1016/s0022-5347(17)50248-7.
We have utilized a human tumor clonogenic assay to grow, and subsequently cytogenetically analyze, tumor colony forming cells from human urologic malignancies. Results following chromosome banding analysis are presented from 4 cases of transitional cell carcinoma and 1 case of renal cell carcinoma. Preliminary evidence suggests a possible association between the loss of chromosome 8 and progression or recurrence of transitional cell carcinoma. Additionally, we have utilized the technique of premature chromosome condensation to identify the interphase chromatin profile of urothelial cells obtained by cystoscopy from 8 patients with transitional cell carcinoma and compared these results to urothelial cells obtained from 7 control patients. This study demonstrates that cells obtained from urologic cancers contain a high proportion of cells in late G1, while normal urothelial cells are usually found in the early G1 phase of the cell cycle. Statistical correlation of premature chromosome condensation analysis suggests this method may be a useful adjunct to routine histopathology in discriminating between normal and cancerous urothelium.
我们利用人类肿瘤克隆形成试验来培养来自人类泌尿生殖系统恶性肿瘤的肿瘤集落形成细胞，并随后对其进行细胞遗传学分析。本文呈现了4例移行细胞癌和1例肾细胞癌经染色体显带分析后的结果。初步证据表明，8号染色体缺失与移行细胞癌的进展或复发之间可能存在关联。此外，我们利用早熟染色体凝集技术来鉴定通过膀胱镜检查从8例移行细胞癌患者获取的尿路上皮细胞的间期染色质图谱，并将这些结果与从7例对照患者获取的尿路上皮细胞进行比较。这项研究表明，泌尿生殖系统癌症来源的细胞在G1期晚期含有高比例的细胞，而正常尿路上皮细胞通常处于细胞周期的G1期早期。早熟染色体凝集分析的统计相关性表明，该方法可能是常规组织病理学在区分正常和癌性尿路上皮方面的有用辅助手段。
