Binggeli R, Weinstein R C
Cancer Res. 1985 Jan;45(1):235-41.
Most cancer cells are known to have lower resting cellular potentials than do their normal counterparts. This study investigates how these potentials establish themselves during growth and cellular contact in tissue culture. Normal quail embryonic fibroblasts and quail fibrosarcoma (QT-35) and normal rat kidney cells and rat fibrosarcoma (from rat fibroblasts chemically transformed by nitroquinoline oxide) were recorded intracellularly using high-impedance micropipets. In high-density high-contact cultures, both quail and rat cancer cells had lower potentials than did normal cells (-20.7 compared to -40.1 mV for quail and -30.7 compared to -61.9 mV for rat). In low-density mitotically synchronous cultures, the rat cells were recorded every 4 hr for 96 hr. Starting at a low density, normal cell membrane potential is maintained at a low level through subsequent cell divisions. Without any additional change in cell density, the potential suddenly elevates to a high level. The membrane potential of cancer cells is by contrast unrelated either to cell density or to time. Cancer cells maintained an intermediate potential from low to very high densities and never elevated their potential to high values. The failure of cancer cells to reach high potentials may be linked to their uncontrolled cell division.
大多数癌细胞的静息细胞电位低于其正常对应细胞。本研究调查了这些电位在组织培养中的生长和细胞接触过程中是如何形成的。使用高阻抗微吸管对正常鹌鹑胚胎成纤维细胞、鹌鹑纤维肉瘤（QT - 35）、正常大鼠肾细胞和大鼠纤维肉瘤（由经硝基喹啉氧化物化学转化的大鼠成纤维细胞形成）进行细胞内记录。在高密度高接触培养中，鹌鹑和大鼠癌细胞的电位均低于正常细胞（鹌鹑细胞分别为 - 20.7 mV和 - 40.1 mV，大鼠细胞分别为 - 30.7 mV和 - 61.9 mV）。在低密度有丝分裂同步培养中，对大鼠细胞每4小时记录一次，共记录96小时。从低密度开始，正常细胞膜电位在随后的细胞分裂过程中保持在较低水平。在细胞密度没有任何额外变化的情况下，电位突然升高到较高水平。相比之下，癌细胞的膜电位与细胞密度或时间均无关。癌细胞在从低密度到非常高密度的过程中保持中等电位，且从未将其电位升高到高值。癌细胞无法达到高电位可能与其不受控制的细胞分裂有关。
