Binggeli R, Cameron I L
Cancer Res. 1980 Jun;40(6):1830-5.
Several lines of investigation point to differences in electrical properties between normal and cancerous cells. Several tumor lines have low-resting membrane potentials. A few comparisons have been made between normal and tumor cells within the same tissue cell type. This study compares the cellular or transmembrane potential of hepatocytes and fibroblasts in both normal and tumor cells. High-impedance micropipets were used to record intracellularly in vivo in Buffalo rat hepatocytes and Morris 7777 hepatoma cells, as well as A/J mouse corneal fibroblasts and poorly differentiated fibrosarcoma cells. Rat hepatocytes had a mean membrane potential of -37.1 +/- 4.3 (S.D.) mV compared to -19.8 +/- 7.1 mV in the hepatoma cells. Mouse corneal fibroblasts measured -42.5 +/- 5.4 mV, while cells of mouse fibrosarcoma were -14.3 +/- 5.4 mV. The membrane potentials of the tumor cells were lower in both instances than in their normal counterpart (statistically significant at p = 0.001 for both tissue cell types). This supports the notion that lower cellular or membrane potentials may play a significant role in the altered physiology of the tumor cell.
多项研究线索表明正常细胞与癌细胞在电特性上存在差异。多种肿瘤细胞系具有较低的静息膜电位。在同一组织细胞类型的正常细胞与肿瘤细胞之间已进行了一些比较。本研究比较了正常细胞和肿瘤细胞中肝细胞和成纤维细胞的细胞内或跨膜电位。使用高阻抗微量移液器在活体状态下对布法罗大鼠肝细胞、莫里斯7777肝癌细胞、A/J小鼠角膜成纤维细胞和低分化纤维肉瘤细胞进行细胞内记录。大鼠肝细胞的平均膜电位为-37.1±4.3（标准差）mV，而肝癌细胞为-19.8±7.1 mV。小鼠角膜成纤维细胞测得为-42.5±5.4 mV，而小鼠纤维肉瘤细胞为-14.3±5.4 mV。在这两种情况下，肿瘤细胞的膜电位均低于其正常对应细胞（两种组织细胞类型在p = 0.001时均具有统计学意义）。这支持了较低的细胞或膜电位可能在肿瘤细胞生理改变中起重要作用这一观点。
