Lysenko V, Guo Y, Rajput V D, Chalenko E, Yadronova O, Zaruba T, Varduny T, Kirichenko E
Academy of Biology and Biotechnology, Southern Federal University, Rostov-on-Don, Russia.
Key Laboratory of Advanced Process Control for Light Industry, Jiangnan University, Wuxi, China.
Photosynthetica. 2023 May 12;61(3):275-284. doi: 10.32615/ps.2023.016. eCollection 2023.
Natural SiO nanoparticles (SiO-NPs) are widely distributed in the environment, and at the same time, synthetic SiO-NP may be applied in agriculture. Evaluations of physiological responses to SiO-NPs treatment of plants are controversial. They are often performed at adaxial leaf sides whereas NPs permeate leaf tissues through stomata located at the abaxial leaf side in the majority of bifacial plants. We measured coefficients of the functional dorsoventral asymmetry of NPs-stressed leaves, S, by values of the CO assimilation rate (S ), dark respiration (S), maximal and operating quantum yields of photosystem II (SF/F, SF'/F'; using PAM-fluorometry), and oxygen coefficients of photosynthesis (SΨ; using photoacoustics). The results indicated that S and SΨ were significantly influenced by SiO-NPs treatment, since and Ψ were declining more markedly when the light was directed to the abaxial side of leaves compared to the adaxial side. Overall, SiO-NPs-induced stress increased 'anoxygenity' of photosynthesis.
天然二氧化硅纳米颗粒（SiO-NPs）广泛分布于环境中，与此同时，合成的SiO-NP可能会应用于农业领域。关于植物经SiO-NPs处理后的生理反应评估存在争议。这些评估通常在叶片近轴面进行，而在大多数双面叶植物中，纳米颗粒是通过位于叶片远轴面的气孔渗透到叶片组织中的。我们通过二氧化碳同化率（S ）、暗呼吸（S）、光系统II的最大和有效量子产率（SF/F，SF'/F'；使用脉冲幅度调制荧光法）以及光合作用的氧系数（SΨ；使用光声法）的值，测量了受纳米颗粒胁迫叶片的功能背腹不对称系数S。结果表明，SiO-NPs处理对S 和SΨ有显著影响，因为与近轴面相比，当光线照射到叶片远轴面时， 和Ψ的下降更为明显。总体而言，SiO-NPs诱导的胁迫增加了光合作用的“无氧性”。
