Academy of Biology and Biotechnology, Southern Federal University, Botanichesky spusk 7, 344041 Rostov-on-Don, Russia.
Funct Plant Biol. 2022 May;49(6):573-586. doi: 10.1071/FP21093.
In contrast to oxygenic photosynthesis, true anoxygenic photosynthesis is not associated with O2 evolution originated from water photolysis but still converts light energy to that of the phosphoanhydride bonds of ATP. In a narrow sense, anoxygenic photosynthesis is mainly known as to be related to the purple and green sulfur bacteria, but in a broad sense, it also occurs in the vascular plants. The portion of photosynthetic water photolysis that is compensated by the processes of O2 uptake (respiration, photorespiration, Mehler cycle, etc.) may be referred to as 'quasi' anoxygenic photosynthesis. Photoacoustic method allows for the separate detection of photolytic O2 at frequencies of measuring light about 20-40Hz, whereas at 250-400Hz, it detects the photochemical energy storage. We have developed a fast-Fourier transform photoacoustic method enabling measurements of both these signals simultaneously in one sample. This method allows to calculate oxygenic coefficients, which reflect the part of photochemically stored light energy that is used for the water photolysis. We show that the true anoxygenic photosynthesis in Ailanthus altissima Mill. leaves reached very high levels under low light, under moderate light at the beginning of the 1-h period, and at the end of the 40-min period under saturating light.
与需氧光合作用相反，真正的厌氧光合作用与水光解产生的 O2 演化无关，但仍将光能转化为磷酸酐键的 ATP。狭义上，厌氧光合作用主要与紫色和绿色硫细菌有关，但广义上，它也发生在维管植物中。光合作用中水光解的一部分被 O2 摄取（呼吸、光呼吸、Mehler 循环等）过程所补偿，可能被称为“准”厌氧光合作用。光声法允许在测量光的频率约为 20-40Hz 时分别检测光解 O2，而在 250-400Hz 时，它可以检测光化学储能。我们开发了一种快速傅里叶变换光声法，能够在一个样品中同时测量这两个信号。该方法允许计算氧系数，该系数反映了用于水光解的光化学储存光能的一部分。我们表明，在低光下，在 1 小时周期的前半段适度光下，以及在饱和光下的 40 分钟周期结束时，臭椿叶片中的真正厌氧光合作用达到了非常高的水平。
