• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

水稻 II 型小分子热激蛋白 OsHsp18.0 在生物和非生物防御反应中均发挥积极作用。

A Class II small heat shock protein OsHsp18.0 plays positive roles in both biotic and abiotic defense responses in rice.

机构信息

College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, Zhejiang, 321004, China.

Hunan Provincial Key Laboratory of Phytohormones and Growth Development, Hunan Agricultural University, Changsha, Hunan, 410128, China.

出版信息

Sci Rep. 2017 Sep 12;7(1):11333. doi: 10.1038/s41598-017-11882-x.

DOI:10.1038/s41598-017-11882-x
PMID:28900229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5595972/
Abstract

Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating diseases of rice. However, the molecular mechanism underpinning the Xoo resistance of rice is still not fully understood. Here, we report that a class II small heat shock protein gene, OsHsp18.0, whose expression was differentially induced between a resistant and a susceptible variety in response to Xoo infection, plays positive roles in both biotic and abiotic resistance. The molecular chaperone activity of OsHsp18.0 was confirmed by a bacterium-expressed glutathione S-transferase fusion protein. Overexpression of OsHsp18.0 in a susceptible rice variety significantly enhanced its resistance to multiple Xoo strains, whereas silencing of OsHsp18.0 in a resistant variety drastically increased its susceptibility. The enhanced Xoo resistance in OsHsp18.0-overexpressing lines was positively correlated with the sensitized salicylic acid-dependent defense responses. In addition to disease resistance, the OsHsp18.0 overexpressing and silencing lines exhibited enhanced and reduced tolerance, respectively, to heat and salt treatments. The subcellular localization study revealed that the green fluorescent protein-OsHsp18.0 was enriched on the nuclear envelope, suggesting a potential role of OsHsp18.0 in the nucleo-cytoplasmic trafficking. Together, our results reveal that the rice OsHsp18.0 is a positive regulator in both biotic and abiotic defense responses.

摘要

由稻黄单胞菌引起的细菌性条斑病（Xoo）是水稻最具破坏性的疾病之一。然而，水稻对 Xoo 抗性的分子机制仍不完全清楚。在这里，我们报告说，一类 II 型小分子热休克蛋白基因 OsHsp18.0，其表达在抗感品种中对 Xoo 感染的反应中存在差异，在生物和非生物抗性中都发挥积极作用。通过细菌表达谷胱甘肽 S-转移酶融合蛋白证实了 OsHsp18.0 的分子伴侣活性。在感病品种中过表达 OsHsp18.0 显著增强了其对多种 Xoo 菌株的抗性，而在抗病品种中沉默 OsHsp18.0 则极大地增加了其敏感性。OsHsp18.0 过表达系增强的 Xoo 抗性与水杨酸依赖的防御反应的敏化呈正相关。除了抗病性外，OsHsp18.0 过表达和沉默系分别表现出对热和盐处理的增强和降低的耐受性。亚细胞定位研究表明，绿色荧光蛋白-OsHsp18.0 富集在核膜上，表明 OsHsp18.0 可能在核质运输中发挥作用。总之，我们的研究结果表明，水稻 OsHsp18.0 是生物和非生物防御反应的正向调节剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/1c0b7777e50c/41598_2017_11882_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/07291f6509c3/41598_2017_11882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/87d15a75401c/41598_2017_11882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/5d026dcf21d2/41598_2017_11882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/3d4683836daf/41598_2017_11882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/560e660fe5dd/41598_2017_11882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/a01d69582ab3/41598_2017_11882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/5f71c2c57294/41598_2017_11882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/1c0b7777e50c/41598_2017_11882_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/07291f6509c3/41598_2017_11882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/87d15a75401c/41598_2017_11882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/5d026dcf21d2/41598_2017_11882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/3d4683836daf/41598_2017_11882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/560e660fe5dd/41598_2017_11882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/a01d69582ab3/41598_2017_11882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/5f71c2c57294/41598_2017_11882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/96bf/5595972/1c0b7777e50c/41598_2017_11882_Fig8_HTML.jpg

相似文献

1
A Class II small heat shock protein OsHsp18.0 plays positive roles in both biotic and abiotic defense responses in rice.水稻 II 型小分子热激蛋白 OsHsp18.0 在生物和非生物防御反应中均发挥积极作用。
Sci Rep. 2017 Sep 12;7(1):11333. doi: 10.1038/s41598-017-11882-x.
2
A pair of allelic WRKY genes play opposite roles in rice-bacteria interactions.一对等位WRKY基因在水稻与细菌的相互作用中发挥相反作用。
Plant Physiol. 2009 Oct;151(2):936-48. doi: 10.1104/pp.109.145623. Epub 2009 Aug 21.
3
OsHsfB4d Binds the Promoter and Regulates the Expression of OsHsp18.0-CI to Resistant Against Xanthomonas Oryzae.OsHsfB4d结合启动子并调控OsHsp18.0-CI的表达以抵抗水稻白叶枯病菌。
Rice (N Y). 2020 May 27;13(1):28. doi: 10.1186/s12284-020-00388-2.
4
Rice OsPAD4 functions differently from Arabidopsis AtPAD4 in host-pathogen interactions.水稻OsPAD4在宿主-病原体相互作用中的功能与拟南芥AtPAD4不同。
Plant J. 2014 May;78(4):619-31. doi: 10.1111/tpj.12500. Epub 2014 Apr 23.
5
JMJ704 positively regulates rice defense response against Xanthomonas oryzae pv. oryzae infection via reducing H3K4me2/3 associated with negative disease resistance regulators.JMJ704通过减少与负向抗病调节因子相关的H3K4me2/3来正向调控水稻对稻瘟病菌感染的防御反应。
BMC Plant Biol. 2015 Dec 9;15:286. doi: 10.1186/s12870-015-0674-3.
6
Abscisic Acid Promotes Susceptibility to the Rice Leaf Blight Pathogen Xanthomonas oryzae pv oryzae by Suppressing Salicylic Acid-Mediated Defenses.脱落酸通过抑制水杨酸介导的防御反应促进水稻对稻瘟病菌的易感性。
PLoS One. 2013 Jun 27;8(6):e67413. doi: 10.1371/journal.pone.0067413. Print 2013.
7
Comparative proteomics reveals differential induction of both biotic and abiotic stress response associated proteins in rice during Xanthomonas oryzae pv. oryzae infection.比较蛋白质组学揭示了水稻在感染稻黄单胞菌稻致病变种期间，与生物和非生物胁迫反应相关的蛋白质的差异诱导情况。
Funct Integr Genomics. 2015 Jul;15(4):425-37. doi: 10.1007/s10142-014-0431-y. Epub 2015 Feb 4.
8
Comparative Transcriptome Analysis of Rice Resistant and Susceptible Genotypes to Xanthomonas oryzae pv. oryzae Identifies Novel Genes to Control Bacterial Leaf Blight.水稻抗和感白叶枯病基因型的比较转录组分析鉴定控制细菌性条斑病的新基因。
Mol Biotechnol. 2021 Aug;63(8):719-731. doi: 10.1007/s12033-021-00338-3. Epub 2021 May 15.
9
OsWRKY67 positively regulates blast and bacteria blight resistance by direct activation of PR genes in rice.OsWRKY67 通过直接激活水稻中的 PR 基因正向调控稻瘟病和细菌性条斑病抗性。
BMC Plant Biol. 2018 Oct 26;18(1):257. doi: 10.1186/s12870-018-1479-y.
10
Overexpression of OsMYC2 Results in the Up-Regulation of Early JA-Rresponsive Genes and Bacterial Blight Resistance in Rice.水稻中OsMYC2的过表达导致早期茉莉酸反应基因上调及对白叶枯病的抗性增强。
Plant Cell Physiol. 2016 Sep;57(9):1814-27. doi: 10.1093/pcp/pcw101. Epub 2016 May 19.

引用本文的文献

1
Identification and Genome Sequencing of Novel Virulent Strains of pv. Causing Rice Bacterial Blight in Zhejiang, China.中国浙江引起水稻白叶枯病的野油菜黄单胞菌水稻致病变种新致病菌株的鉴定与基因组测序
Pathogens. 2024 Dec 9;13(12):1083. doi: 10.3390/pathogens13121083.
2
Transcriptomics and metabolomics analyses of Rosa hybrida to identify heat stress response genes and metabolite pathways.转录组学和代谢组学分析杂种玫瑰以鉴定热应激响应基因和代谢途径。
BMC Plant Biol. 2024 Sep 20;24(1):874. doi: 10.1186/s12870-024-05543-1.
3
Regulatory network of rice in response to heat stress and its potential application in breeding strategy.

本文引用的文献

1
Prospects of engineering thermotolerance in crops through modulation of heat stress transcription factor and heat shock protein networks.通过调节热应激转录因子和热休克蛋白网络提高作物耐热性的前景。
Plant Cell Environ. 2015 Sep;38(9):1881-95. doi: 10.1111/pce.12396. Epub 2014 Aug 6.
2
A positive feedback loop between HEAT SHOCK PROTEIN101 and HEAT STRESS-ASSOCIATED 32-KD PROTEIN modulates long-term acquired thermotolerance illustrating diverse heat stress responses in rice varieties.热激蛋白101与热胁迫相关32-kD蛋白之间的正反馈回路调节长期获得性耐热性，这说明了水稻品种中不同的热胁迫反应。
Plant Physiol. 2014 Apr;164(4):2045-53. doi: 10.1104/pp.113.229609. Epub 2014 Feb 11.
3
水稻响应热胁迫的调控网络及其在育种策略中的潜在应用
Mol Breed. 2023 Aug 21;43(9):68. doi: 10.1007/s11032-023-01415-y. eCollection 2023 Sep.
4
Pan-transcriptomic Profiling Demarcates Serendipita Indica-Phosphorus Mediated Tolerance Mechanisms in Rice Exposed to Arsenic Toxicity.全转录组分析揭示了印度梨形孢-磷介导的水稻对砷毒性的耐受机制。
Rice (N Y). 2023 Jun 24;16(1):28. doi: 10.1186/s12284-023-00645-0.
5
Identification of simple sequence repeat markers linked to heat tolerance in rice using bulked segregant analysis in F population of NERICA-L 44 × Uma.利用NERICA-L 44×Uma杂交F群体通过集团分离分析法鉴定与水稻耐热性相关的简单序列重复标记。
Front Plant Sci. 2023 Mar 27;14:1113838. doi: 10.3389/fpls.2023.1113838. eCollection 2023.
6
Gene Networks Involved in Plant Heat Stress Response and Tolerance.植物热应激响应和耐受相关的基因网络。
Int J Mol Sci. 2022 Oct 9;23(19):11970. doi: 10.3390/ijms231911970.
7
Engineering plant immune circuit: walking to the bright future with a novel toolbox.工程植物免疫回路：用新型工具箱走向光明未来。
Plant Biotechnol J. 2023 Jan;21(1):17-45. doi: 10.1111/pbi.13916. Epub 2022 Sep 27.
8
A maize heat shock factor ZmHsf11 negatively regulates heat stress tolerance in transgenic plants.ZmHsf11，一种玉米热激因子，负调控转基因植株的耐热性。
BMC Plant Biol. 2022 Aug 20;22(1):406. doi: 10.1186/s12870-022-03789-1.
9
Soybean F-Box-Like Protein GmFBL144 Interacts With Small Heat Shock Protein and Negatively Regulates Plant Drought Stress Tolerance.大豆类F-Box蛋白GmFBL144与小分子热激蛋白相互作用并负向调控植物耐旱胁迫耐受性。
Front Plant Sci. 2022 Jun 2;13:823529. doi: 10.3389/fpls.2022.823529. eCollection 2022.
10
A review of approaches to control bacterial leaf blight in rice.水稻细菌性条斑病防治方法综述。
World J Microbiol Biotechnol. 2022 May 17;38(7):113. doi: 10.1007/s11274-022-03298-1.
Chaperone function and mechanism of small heat-shock proteins.
伴侣蛋白的功能和小热休克蛋白的作用机制。
Acta Biochim Biophys Sin (Shanghai). 2014 May;46(5):347-56. doi: 10.1093/abbs/gmt152. Epub 2014 Jan 20.
4
Roles of plant hormones and their interplay in rice immunity.植物激素的作用及其在水稻免疫中的相互作用。
Mol Plant. 2013 May;6(3):675-85. doi: 10.1093/mp/sst056. Epub 2013 Apr 15.
5
An Arabidopsis homolog of importin β1 is required for ABA response and drought tolerance.拟南芥 Importin β1 同源物是 ABA 响应和抗旱性所必需的。
Plant J. 2013 Aug;75(3):377-89. doi: 10.1111/tpj.12207. Epub 2013 May 13.
6
Rice versus Xanthomonas oryzae pv. oryzae: a unique pathosystem.水稻与稻黄单胞菌：一个独特的病理系统。
Curr Opin Plant Biol. 2013 May;16(2):188-95. doi: 10.1016/j.pbi.2013.02.008. Epub 2013 Mar 4.
7
An unusual dimeric small heat shock protein provides insight into the mechanism of this class of chaperones.一种不寻常的二聚体小分子热休克蛋白为这类伴侣蛋白的作用机制提供了深入了解。
J Mol Biol. 2013 May 27;425(10):1683-96. doi: 10.1016/j.jmb.2013.02.011. Epub 2013 Feb 14.
8
Overexpression of a soybean nuclear localized type-III DnaJ domain-containing HSP40 reveals its roles in cell death and disease resistance.过量表达一种大豆核定位的 III 型 DnaJ 结构域热休克蛋白 40 揭示了其在细胞死亡和抗病性中的作用。
Plant J. 2013 Apr;74(1):110-21. doi: 10.1111/tpj.12108. Epub 2013 Feb 18.
9
The evolution, function, structure, and expression of the plant sHSPs.植物 sHSPs 的进化、功能、结构和表达。
J Exp Bot. 2013 Jan;64(2):391-403. doi: 10.1093/jxb/ers355. Epub 2012 Dec 18.
10
The Opuntia streptacantha OpsHSP18 gene confers salt and osmotic stress tolerance in Arabidopsis thaliana.仙人掌链刺仙人掌OpsHSP18基因赋予拟南芥耐盐和渗透胁迫能力。
Int J Mol Sci. 2012;13(8):10154-10175. doi: 10.3390/ijms130810154. Epub 2012 Aug 15.