Chen Haipeng, Chen Xiaolin, Li Xiaogang, Lin Xin, Yue Lihua, Liu Chunhai, Li Yuling
College of Forestry, Hebei Agricultural University, Baoding, Hebei, China.
Technical Center, Chengde Astronaut Mountainous Plant Technology Co. Ltd. Chengde, Hebei, China.
Plant Signal Behav. 2024 Dec 31;19(1):2439256. doi: 10.1080/15592324.2024.2439256. Epub 2024 Dec 9.
) is the primary tree species known for its ecological and economic benefits in arid and semi-arid regions. Understanding the response of roots to drought stress is essential for promoting the development of varieties. One-year-old Yulu was utilized as the experimental material, and three water gradients were established: control (CK), moderate (T1) and severe (T2), over a period of 120 days. The phenotypic traits and physiological indies were assessed and analyzed, while the roots were subjected by RNA-Seq transcriptome and Tandem Mass Tags (TMT) proteome analysis. Drought stress significantly reduced the plant height, ground diameter, root biomass and superoxide dismutase activity; however, the main root length increased. In comparison with CK, a total of 5789 and 5594 differential genes, as well as 63 and 1012 differential proteins, were identified in T1 and T2, respectively. The combined analysis of transcriptome and proteome showed that the number of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) associated with T1, T2 and CK was 28 and 126, respectively, with 7 and 36 genes achieving effective KEGG annotation. In T1 and T2, the differential genes were significantly enriched in the plant hormone signal transduction pathway, but there was no significant enrichment in the protein expression profile. In T2, 38 plant hormone signal transduction function genes and 10 peroxisome related genes were identified. With the increase of drought stress, the combined expression of DEGs and DEPs increased. Yulu may allocate more resources toward CAT while simultaneously decreasing SOD and POD to mitigate the oxidative stress induced by drought. Furthermore, the molecular mechanisms underlying plant hormone signal transduction and peroxisome-related genes in the roots of were discussed in greater detail.
（某树种）是干旱和半干旱地区以其生态和经济效益而闻名的主要树种。了解（该树种）根系对干旱胁迫的响应对于促进品种发展至关重要。以一年生的雨露（音译，可能是某个特定品种名）作为实验材料，在120天的时间里建立了三个水分梯度：对照（CK）、中度（T1）和重度（T2）。对表型性状和生理指标进行了评估和分析，同时对根系进行了RNA测序转录组和串联质谱标签（TMT）蛋白质组分析。干旱胁迫显著降低了株高、地径、根生物量和超氧化物歧化酶活性；然而，主根长度增加。与CK相比，在T1和T2中分别鉴定出总共5789个和5594个差异基因，以及63个和1012个差异蛋白质。转录组和蛋白质组的联合分析表明，与T1、T2和CK相关的差异表达基因（DEGs）和差异表达蛋白质（DEPs）的数量分别为28个和126个，其中7个和36个基因实现了有效的KEGG注释。在T1和T2中，差异基因在植物激素信号转导途径中显著富集，但在蛋白质表达谱中没有显著富集。在T2中，鉴定出38个植物激素信号转导功能基因和10个过氧化物酶体相关基因。随着干旱胁迫的增加，DEGs和DEPs的联合表达增加。雨露（音译）可能会将更多资源分配给过氧化氢酶（CAT），同时降低超氧化物歧化酶（SOD）和过氧化物酶（POD），以减轻干旱诱导的氧化应激。此外，还更详细地讨论了该树种根系中植物激素信号转导和过氧化物酶体相关基因的分子机制。
